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目的探讨应用实时定量聚合酶链反应(RQ-PCR)法对异基因造血干细胞移植(allo- HSCT)后巨细胞病毒(CMV)感染的诊断价值及对临床用药的指导意义。方法33例行allo-HSCT、的患者于造血重建后,应用RQ-PCR法对其外周血CMV DNA进行动态检测,根据检测结果决定抗CMV治疗的开始、减量和终止,观察治疗效果和临床转归。结果共检出13例CMV感染患者,21次感染发作；除1次是患者中途放弃治疗外,余20次治疗中CMV DNA拷贝迅速转阴或下降至转阴,有临床表现者症状消失、器官功能恢复；且抗CMV疗程短于常规疗程。结论应用RQ-PCR法不但可以早期诊断CMV感染,及时干预治疗,还可以直观指导治疗的减量和终止、缩短疗程、减轻药物不良反应。
Objective To investigate the diagnostic value of real-time quantitative polymerase chain reaction (RQ-PCR) for cytomegalovirus (CMV) infection after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and its clinical significance. Methods Thirty-three allo-HSCT patients underwent hematopoietic reconstitution. RQ-PCR was used to detect the CMV DNA in peripheral blood. The onset, reduction and termination of anti-CMV treatment were determined according to the test results. The therapeutic effect and clinical Go. Results A total of 13 cases of CMV infection were detected and 21 infections were detected. Except for 1 case, patients gave up treatment halfway and CMV DNA copies rapidly turned negative or decreased to negative after more than 20 treatments. The symptoms of clinical manifestations disappeared. Functional recovery; and anti-CMV treatment shorter than the conventional course of treatment. Conclusion The RQ-PCR method can not only early diagnosis of CMV infection, timely intervention, but also can directly guide the treatment of reduction and termination, shorten the course of treatment, reduce adverse drug reactions.