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采用A-PAGE和SDS-PAGE方法,对来自不同地方的23个抗赤霉病小麦地方品种的醇溶蛋白和谷蛋白亚基进行了分析。结果表明,在A-PAGE电泳分析中,23个供试品种具有23种不同的醇溶蛋白带型,共分离出39条相对迁移率不同的谱带,其中31条具有多态性,占86.8％,每份材料可电泳出14～23条带,存在着广泛的等位基因变异。在SDS-PAGE电泳分析中,出现了9种不同的高分子量谷蛋白亚基及6种亚基组合类型,优质亚基及亚基组合所占的比例较少,品质评分较低,其变幅为5～9分,平均为6.8分。
The gliadin and glutelin subunits of 23 wheat cultivars with different resistance to Fusarium head blight were analyzed by A-PAGE and SDS-PAGE. The results showed that in the A-PAGE electrophoresis analysis, 23 different gliadin bands were detected in 23 cultivars, and 39 bands with different relative mobility were isolated, of which 31 were polymorphic, accounting for 86.8 %, Each material can be electrophoresed 14 to 23 bands, there is a wide range of allelic variation. In the SDS-PAGE electrophoresis analysis, there are nine different types of high molecular weight glutenin subunits and combinations of six subtypes, the proportion of high quality subunit and subunit combination accounted for less, the quality score is lower, the amplitude For 5 to 9 points, an average of 6.8 points.