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目的观察α-促黑素(α-MSH)对小鼠骨骼肌细胞脂肪酸氧化的影响及其机制。方法 8周龄C57BL/6J小鼠分成生理盐水对照组和实验组(α-MSH组),两组分别皮下注射生理盐水和α-MSH后0、0.5、1、3、5h测定比目鱼肌的脂肪酸氧化限速酶-肉毒碱棕榈酰转移酶-1(CPT-1)活性。体外培养的原代骨骼肌细胞和C2C12细胞,利用RT-PCR法测定黑皮质素受体(MCR)的表达,并α-MSH处理2h后观察脂肪酸氧化。结果α-MSH皮下注射后1、3h时CPT-1活性显著增加,与对照组相比差异有统计学意义(P<0.05)。原代骨骼肌细胞和C2C12细胞中表达MC1R、MC3R、MC4R和MC5R,α-MSH剂量依赖性地促进脂肪酸氧化。结论α-MSH可能通过MCR促进骨骼肌细胞脂肪酸氧化。
Objective To investigate the effect of α-melanin (α-MSH) on fatty acid oxidation in mouse skeletal muscle cells and its mechanism. Methods 8-week-old C57BL / 6J mice were divided into saline control group and experimental group (α-MSH group). The fatty acids of soleus muscle were measured at 0, 0.5, 1, 3 and 5 h after subcutaneous injection of normal saline and α-MSH Oxidative rate-limiting enzyme - Carnitine palmitoyltransferase-1 (CPT-1) activity. The primary cultured skeletal muscle cells and C2C12 cells were cultured in vitro. The expression of melanocortin receptor (MCR) was measured by RT-PCR and the fatty acid oxidation was observed after treated with α-MSH for 2 hours. Results The activity of CPT-1 at 1 and 3 hours after α-MSH subcutaneous injection was significantly increased compared with the control group (P <0.05). Primary skeletal muscle cells and C2C12 cells express MC1R, MC3R, MC4R and MC5R, and α-MSH dose-dependently promote fatty acid oxidation. Conclusion α-MSH may promote the fatty acid oxidation of skeletal muscle cells through MCR.