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目的研究白细胞介素-6(IL-6)(-634C/G)基因多态性与尘肺的关系。方法选择确诊的104例Ⅰ期男性矽肺患者66例(矽肺组)、煤工尘肺患者38例(煤工尘肺组)为研究对象,以接触同性质粉尘、非尘肺的122名男性接触矽尘工人77例(矽尘组)、接触煤尘工人45例(煤尘组)为对照；采集外周静脉血,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测IL-6(-634C/G)基因多态性。结果IL-6(-634C/G)基因型(CC、CG、GG)在矽肺组、矽尘组、煤工尘肺组和煤尘组分布频率分别为66．7%、19．7%、13．6%,42．9%、42．9%、14．2%,73．7%、18．4%、7．9%和51．1%、35．65、13．3%。成组分析和1∶1配对分析中均发现IL-6(-634C/G)基因型分布频率在矽肺组和矽尘组间差异有统计学意义(P＜0．05)。结论IL-6(-634C/G)基因多态性与矽肺发病相关。
Objective To study the relationship between interleukin-6 (IL-6) (-634C / G) gene polymorphism and pneumoconiosis. Methods Sixty-four cases of silicosis Ⅰ (silicosis group) were enrolled in 104 cases of Ⅰ stage silicosis patients and 38 cases of coal worker’s pneumoconiosis (coal workers’ pneumoconiosis group). 122 male workers with the same type of dust and non-pneumoconiosis were exposed to silica dust workers 77 cases (silica dust group) and 45 workers exposed to coal dust (coal dust group) were taken as control. Peripheral venous blood was collected and detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) (-634C / G) gene polymorphism. Results The frequencies of IL-6 (-634C / G) genotypes (CC, CG, GG) were 66.7%, 19.7%, 13.7% in silicosis group, .6%, 42.9%, 42.9%, 14.2%, 73.7%, 18.4%, 7.9% and 51.1%, 35.65, and 13.3% respectively. The frequency of IL-6 (-634C / G) genotype was found to be significantly different between the silicosis group and the silicagel group (P <0.05) in the group analysis and the 1: 1 paired analysis. Conclusion IL-6 (-634C / G) gene polymorphism is associated with silicosis.