Combining the temperature and precipitation data from 77 climatological stations and the climatic and hydrological change data from three headstreams of the Tarim River: Hotan, Yarkant, and Aksu in the study area, the plausible association between climate change and the variability of water resources in the Tarim River Basin in recent years was investigated, the long-term trend of the hydrological time series including temperature, precipitation, and stream-flow was detected, and the possible association between the El Nino/Southern Oscillation (ENSO) and these three kinds of time series was tested. The results obtained in this study show that during the past years, the temperature experienced a significant monotonic increase at the speed of 5%, nearly 1℃rise; the precipitation showed a significant decrease in the 1970s, and a significant increase in the 1980s and 1990s, the average annual precipitation was increased with the magnitude of 6.8 mm per decade. A step change occurred in both temperature and Combining the temperature and precipitation data from 77 climatological stations and the climatic and hydrological change data from three headstreams of the Tarim River: Hotan, Yarkant, and Aksu in the study area, the plausible association between climate change and the variability of water resources in the Tarim River Basin in recent years was investigated, the long-term trend of the hydrological time series including temperature, precipitation, and stream-flow was detected, and the possible association between the El Nino / Southern Oscillation (ENSO) and these three kinds of time series was tested. The results obtained in this study show that during the past years, the temperature experienced a significant monotonic increase at the speed of 5%, nearly 1 ℃ rise; the precipitation showed a significant decrease in the 1970s, and a significant increase in the 1980s and 1990s, the average annual precipitation increased with the magnitude of 6.8 mm per decade. A step change occurred in both temperature and