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长期以来,有性杂交是被广泛应用和卓有成效的育种方法。然而,远缘植物之间由于不亲和性等原因,往往杂交不孕或杂种不育。数十年前,人们就设想并着手研究用体细胞杂交的方法,来克服这种天然障碍,进而根据人类的不同需要,广泛地组合各种基因型,创造崭新的生物类型或品种。近十年来,伴随着植物原生质体游离和培养技术的迅速提高,植物体细胞杂交研究非常活跃,进展很快,不仅在原生质体融合及其筛选技术上有显著改进,而且
For a long time, sexual hybridization is a widely used and fruitful breeding method. However, due to incompatibility between distant plants and other reasons, often infertility or hybrid sterility. Decades ago, people conceived and started to study the method of somatic cell hybridization to overcome this natural obstacle, and then, based on the different needs of human beings, a wide range of genotypes were combined to create a brand new biological type or variety. In recent ten years, along with the rapid increase of plant protoplast dissociation and culture technology, somatic cell hybridization research is very active and rapid progress, not only in the protoplast fusion and screening technology significantly improved, and