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水稻的穗形与其产量关系密切,也是研究的一大热点。利用60Co-γ射线辐射诱变水稻68902B,筛选到一个水稻稀穗突变体,暂命名为Oslp(Oryza sativa lax panicle)。研究了该突变体的主要农艺性状与稀穗的遗传方式,并对稀穗突变基因Oslp进行了分子定位。结果显示稀穗突变体Oslp的每穗粒数为104粒、二次枝梗数目为7个,它们都显著地少于原品种68902B每穗粒数的124粒和二次枝梗数目的 20个。遗传分析揭示突变体Oslp的稀穗性状受一对隐性核基因控制。将(籼稻品种261S×稀穗突变体Oslp)F2代中的稀穗个体作为定位群体,结合BSA和SSR分子标记技术,将基因Oslp定位在第7号染色体短臂的2个分子标记FR-3和FR-4之间,基因Oslp与FR-3和FR-4的遗传距离分别为0.6 c M和0.8 c M。
The spike shape of rice is closely related to its yield and is also a hot spot of research. A rice ear mutant was screened by 60Co-γ-ray irradiation of rice 68902B and temporarily named as Oslp (Oryza sativa lax panicle). The main agronomic traits and spike inheritance of this mutant were studied, and the molecular mutation of Oslp gene was studied. The results showed that there were 104 spikelets per spike and 7 secondary spikelets in the spike mutant Oslp, which were significantly less than 124 spores per spike of the original cultivar 68902B and 20 secondary branches. Genetic analysis revealed that the spike trait of mutant Oslp was controlled by a recessive nuclear gene. The spike individuals from F2 generation of indica rice cultivar 261S × spike mutant Oslp were used as locating groups, and combined with BSA and SSR molecular markers, the gene Oslp was mapped on the two molecular markers FR-3 And FR-4, the genetic distances between Oslp and FR-3 and FR-4 were 0.6 cM and 0.8 cM, respectively.