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目的探讨甘肃地区PRKAA2基因rs2746342(G/T)单核苷酸多态性与T2DM及血清脂联素(APN)、抵抗素(Resistin)的关系。方法甘肃地区T2DM患者163例和正常对照组86名,两组年龄、性别匹配,采用聚合酶链反应/DNA限制性片段长度多态性(PCR/RFLP)、酶联免疫分析(ELISA)检测其基因多态性、空腹C肽(FCP)、APN、Resistin,同时测身高、体重、血压、BMI、空腹血糖、血脂等。结果 (1)T2DM组与正常对照组的基因型和等位基因频度分布存在显著差异(P<0.05);(2)T2DM组各基因型T/T、G/T、G/G中Resistin、TG、TC、LDL-C、FC-P依次降低,APN、HDL-C依次增高(P<0.05);(3)APN水平在糖尿病超重组低于糖尿病正常体重组和对照组,Resistin水平在糖尿病超重组高于糖尿病正常体重组和对照组(P<0.05);(4)相关分析表明APN水平与BMI、血压负相关,与C肽正相关(P<0.05),Resistin水平与BMI、血压正相关,与C肽负相关(P<0.05);(5)T2DM超重组较T2DM正常体重组和正常对照组TG、LDL-C水平升高(P<0.05),HDLC水平降低(P<0.05)。结论甘肃地区PRKAA2基因rs2746342(G/T)单核苷酸基因多态性可能与T2DM及胰岛素抵抗、血清APN、Re-sistin水平关联。
Objective To investigate the association of rs2746342 (G / T) single nucleotide polymorphisms in PRKAA2 gene with T2DM, serum adiponectin (APN) and resistin (Resistin) in Gansu province. Methods Totally 163 patients with T2DM in Gansu province and 86 normal controls were enrolled in this study. The age and sex of the two groups were matched. PCR / RFLP and enzyme-linked immunosorbent assay (ELISA) Genetic polymorphisms, fasting C-peptide (FCP), APN, Resistin, height, weight, blood pressure, BMI, fasting blood glucose, Results (1) There was significant difference in genotype and allele frequency distribution between T2DM group and normal control group (P <0.05). (2) Resistin in T / T, G / T and G / G of T2DM group (P <0.05); (3) The level of APN in diabetic overweight group was lower than that of normal weight group and control group, and the level of Resistin was (4) The correlation analysis showed that the level of APN was negatively correlated with BMI and blood pressure, positively correlated with C peptide (P <0.05), and the level of Resistin was positively correlated with BMI, blood pressure (P <0.05); (5) The levels of TG and LDL-C in T2DM overweight group were significantly higher than those in T2DM normal control group and normal control group (P <0.05), HDLC level was lower (P <0.05) ). Conclusion The single nucleotide polymorphism of rs2746342 (G / T) in PRKAA2 gene in Gansu province may be related to the level of insulin resistance, APN and Re-sistin in T2DM.