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本实验用Wistar大鼠建立三种脑损伤模型，即脑顶叶皮层针刺损伤、全脑缺血／再灌注和急性高原（5000米）缺氧，应用胶质原纤维酸性蛋白（GFAP）单克隆抗体免疫组化方法，观察了伤后1天，3天，5天，7天，10天，14天，21天皮层、海马及室周区星形胶质细胞的形态学变化。结果如下：（1）针刺脑损伤组：术后5－7天，伤侧海马CAl、CA。区及室周区可见星形胶质细胞染色加深，胞体增大，数目增加，伤道底部GFAP阳性纤维长短不一；至14天左右阳性细胞突起增长增粗，数目进一步增加，伤道底部阳性纤维横向排列，多垂直于伤道。（2）全脑缺血／再灌注组：术后1天即可见海马CA；、CA。区星形胶质细胞染色增强，胞体增大；术后3－5天，皮层、海马CAl、CA。区及室周区星形胶质细胞脑体增大，突起增粮、增长，染色加深，细胞数目产多，海马齿状回处GFAP阳性纤维较正常增多增长，且呈辐射状排列：水后14－ZI天，仍见上述反应，但无进一步改变。（3）急性高原缺氧组；1—5天，星形胶质细胞未见明显形态学变化；10—14天，可见有轻微的GFAP阳性细胞数目增多及少数细胞胞体增大。
In this study, we established three models of brain injury in Wistar rats: acupuncture injury of cerebral parietal cortex, global ischemia / reperfusion and hypoxia in acute plateau (5000 m). Using glial fibrillary acidic protein (GFAP) The morphological changes of astrocytes in the cortex, hippocampus and periventricular zone were observed at 1 day, 3 days, 5 days, 7 days, 10 days, 14 days and 21 days after injury by immunohistochemistry. The results are as follows: (1) acupuncture brain injury group: 5-7 days after injury, injured side of the hippocampus CAl, CA. In the region and periventricular zone, the staining of astrocytes deepened, the number of cell bodies increased and the number increased. The length of GFAP positive fibers at the bottom of the wounds varied from 14 days to 14 days. The number of positive cells increased and the number increased further Fiber horizontal arrangement, more perpendicular to the injury. (2) Ischemia / reperfusion group: hippocampal CA; District astrocyte staining enhanced cell body increased 3-5 days after cortex, hippocampus CAl, CA. The number of astrocyte in the area and periventricular zone increased, the number of neurons in the hippocampal dentate gyrus increased, the number of neurons in the hippocampus increased, the number of astrocytes increased, The above reaction is still seen on day 14-ZI, but without further changes. (3) Acute plateau hypoxia group; 1-5 days, no obvious morphological changes of astrocytes; 10-14 days, showing a slight increase in the number of GFAP positive cells and a small number of cells increased.