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目的:研究低氘环境对人胃癌细胞(SGC-7901)增殖的影响并初步探讨其相关机制。方法:用含不同氘浓度的蒸馏水(实验组:25 ppm;对照组:150 ppm)配制的RPMI-1640培养基培养胃癌细胞SGC-7901。分别在不同的时间点对两组细胞的增殖率、细胞周期及凋亡情况进行检测,用Western blot法对两组细胞的增殖细胞核抗原蛋白(PCNA)的表达进行检测。结果:低氘环境下SGC-7901细胞的增殖率比对照组低10%左右。低氘环境对细胞的划痕愈合能力及集落形成能力也有显著抑制作用(P<0.05)。流式细胞术检测结果显示,与对照组相比,低氘组的细胞G1期细胞的比例增加(P<0.01),而其所处S期细胞的比例下降(P<0.05),两组细胞间早凋及晚凋比率差异无统计学意义。Western blot的结果显示低氘环境下培养的胃癌细胞的PCNA的表达明显下降。结论:低氘环境能够抑制胃癌细胞的生长,这可能与低氘环境下胃癌细胞阻滞于G1期及下调其PCNA的表达有关。
Objective: To study the effect of low deuterium environment on the proliferation of human gastric cancer cell line SGC-7901 and to explore its underlying mechanism. Methods: Gastric cancer cells SGC-7901 were cultured in RPMI-1640 medium containing different deuterium concentrations in distilled water (experimental group: 25 ppm; control group: 150 ppm). The proliferation, cell cycle and apoptosis of the two groups of cells were detected at different time points. The expression of proliferating cell nuclear antigen protein (PCNA) was detected by Western blot. Results: The proliferation rate of SGC-7901 cells in deuterium-depleted environment was about 10% lower than that of the control group. Low-deuterium environment also significantly inhibited the healing of scratches and colony-forming ability of cells (P <0.05). The results of flow cytometry showed that the percentage of cells in G1 phase in low deuterium group increased (P <0.01), and the proportion of S phase cells in S phase decreased (P <0.05) compared with that in control group There was no significant difference between early withered and late withered. Western blot results showed that the expression of PCNA in gastric cancer cells cultured in low deuterium environment decreased significantly. CONCLUSION: Low deuterium environment can inhibit the growth of gastric cancer cells, which may be related to the cell cycle arrest of G1 phase and the down-regulation of PCNA expression under low deuterium environment.