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从病毒感染液的制备，围栏集虫感染增殖病毒，适时采收，整虫提取ＣＰＶ等方面详细地介绍了松毛虫细胞质型多角体病毒的围栏增殖方法，并就这种方法涉及到病毒增殖效果的集虫虫质、增殖时机、接种浓度、感病虫的鉴别和病毒产量的预测等方面进行分析，阐明采用此方法较好地解决了大量增殖病毒的一系列技术性问题，具有增殖量大、产品质量好、生产成本低、适合我国国情的优点，达到病虫回收率超过８０％，单虫病毒多角体产量大于２亿的要求。
From the preparation of the virus infection liquid, the infection of the penicillium contagiosum, the timely harvest and the complete CPV extraction, the method of enclosure multiplication of the cytoplasmic polyhedrosis virus of the pine caterpillars was introduced in detail. In this method, the virus proliferation effect Of the collection of insects quality, timing of proliferation, inoculation concentration, the identification of susceptible pests and the prediction of the virus yield and other aspects to clarify the use of this method to solve a large number of proliferative viruses a series of technical problems, with a large amount of proliferation, Good product quality, low production cost, suitable for the advantages of our national conditions, pest recovery rate of more than 80%, single worm polyhedron production of more than 200 million requirements.