利用吸附法，将圆柱状假丝酵母脂肪酶固定于４种疏水性不同的载体上．固定化酶的活性及稳定性随载体疏水性的增大而增大．用ＹＷＧ－Ｃ６Ｈ５作为载体，在有机溶剂－水双液相体系中催化萘普生甲酯的不对称水解，反应１２０ｈ转化率为２４．７２％，产品的对映体过量值（ｅｅｐ）为９４．８２％．并发现添加乳糖有助于提高固定化酶的稳定性，固定化酶批式连续反应半衰期达１０００ｈ以上． Using the adsorption method, Candida cylindracea lipase was immobilized on four different hydrophobic carriers. Immobilized enzyme activity and stability with the carrier hydrophobic increases. The asymmetric hydrolysis of naproxen methyl ester was catalyzed by YWG-C6H5 as carrier in organic solvent-water two-liquid system. The reaction yield was 24.72% after 120 h and the enantiomeric excess (eep) was 94 .82%. And found that adding lactose helps to improve the stability of immobilized enzyme, immobilized enzyme batch continuous reaction half-life of more than 1000h.