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目的探讨血清硅元素水平在矽肺发病机制中的意义和用于矽肺早期诊断的价值。方法采用电感耦合等离子体质谱仪(ICP-MS)检测矽肺模型大鼠血清中硅元素水平,同时采用免疫组织化学-SP法观察转化生长因子-β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)在大鼠矽肺模型肺组织的表达。结果矽肺模型大鼠染尘后血清硅元素水平明显增高,1d内增长高峰为16h;其后的7、14、21和28d,其水平亦显著高于对照组,差异有统计学意义(P<0.05)。大鼠染尘后肺组织TGF-β1表达与对照组比较,差异有统计学意义(P<0.05),表达高峰是染尘后的第7天;TNF-α表达与对照组比较,在染尘后的7、14、21和28d显著高于对照组,差异有统计学意义(P<0.05),表达高峰为染矽尘后的第21天。结论矽肺模型大鼠血清硅元素水平早期即明显升高且早于TGF-β1、TNF-α的表达,血清硅元素水平变化可以作为矽肺的早期诊断指标。
Objective To investigate the significance of serum silicon in the pathogenesis of silicosis and its value in the early diagnosis of silicosis. Methods The levels of silicon in the serum of silicotic model rats were determined by inductively coupled plasma mass spectrometry (ICP-MS), and the expressions of transforming growth factor-β1 (TGF-β1) and tumor necrosis factor-α TNF-α) in the lung of rat model of silicosis. Results The levels of serum silicon in the silicosis model rats were significantly increased after the dust was dusted, with a peak of 16h after the first day. The levels of silicon in the silicosis model group were significantly higher than those of the control group at 7, 14, 21 and 28 days (P < 0.05). Compared with the control group, the expression of TGF-β1 in the lung tissue of the rats was significantly different (P <0.05), and the peak of expression of TGF-β1 was the 7th day after the dust was dyed. Compared with the control group, After 7, 14, 21 and 28 d, the expression was significantly higher than that of the control group (P <0.05). The peak of expression was on the 21st day after exposure to silica dust. Conclusion The level of serum silicon in silicosis model rats increased significantly early and earlier than that of TGF-β1 and TNF-α, and the changes of serum silicon levels could be used as an early diagnostic index for silicosis.