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目的检测恶性黑素瘤标本中的端粒酶活性,探讨端粒酶在黑素瘤发生机制中的作用。方法采用聚合酶链反应-酶联免疫吸附测定法(PCR-ELISA),对黑素瘤标本进行端粒酶的定性和定量分析。结果90.0％的原发性黑素瘤和100％的转移性黑素瘤显示端粒酶阳性,仅14.3％的黑素细胞痣呈阳性,而正常皮肤中均为阴性。转移性黑素瘤、原发性黑素瘤、黑素细胞痣和正常皮肤的端粒酶活性水平的平均A值分别为0.729、0.405、0.118和0.044。各组端粒酶活性A值之间经统计学分析差异有显著性。结论大部分恶性黑素瘤患者中存在高水平的端粒酶活性,提示端粒酶可能在黑素瘤的发生机制中起重要作用,对黑素瘤的诊断有一定的临床意义。
Objective To detect the telomerase activity in malignant melanoma specimens and to explore the role of telomerase in melanoma pathogenesis. Methods Polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA) was used to qualitatively and quantitatively detect telomerase in melanoma specimens. Results 90.0% of primary melanoma and 100% of metastatic melanoma showed telomerase positive, only 14.3% of melanocytic nevus was positive, while normal skin was negative. Mean A values for telomerase activity levels of metastatic melanoma, primary melanoma, melanocytic nevus and normal skin were 0.729, 0.405, 0.188 and 0.044, respectively. The differences of telomerase activity A between each group were statistically significant. Conclusions High levels of telomerase activity exist in most malignant melanoma patients, suggesting that telomerase may play an important role in the pathogenesis of melanoma and have certain clinical significance for the diagnosis of melanoma.