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稻瘟病是稻瘟菌引起的一种世界性病害,每年造成水稻大幅减产。真菌病毒是指能够在真菌体内进行复制和繁殖的病毒,可以作为生物防治的资源。本实验从发病的水稻叶片上采用单孢分离的方法获得纯化培养的稻瘟菌菌株YC13。菌株YC13携带多条dsRNA片段,其中片段F1与Magnaporthe oryzae virus 2(MoV2)的Coat Protein(CP)和RNA-dependent RNA polymerase(RdRp)的氨基酸序列均具有99%的同源性,确定为MoV2的基因组;片段F4、F5序列与双分病毒Penicillium stoloniferum virus F(Ps V-F)的RdRp和CP的氨基酸序列分别具有69%和63%的同源性,命名为Magnaporthe oryzae partitivirus virus 2(MoPV2)。MoPV2的基因组含有2条dsRNA:dsRNA 1编码RdRp,dsRNA 2编码CP。根据MoPV2的RdRp编码的氨基酸序列将MoPV2归属于双分病毒科(Partitiviridae)G ammapartitivirus属,是一种新的真菌病毒。通过病毒粒子转染,将MoPV2转到稻瘟菌菌株RB11。与菌株RB11相比,带毒菌株RB11T25的分生孢子产生量显著减少,并且对大麦活体的致病性也有所降低,但其他生物学特征均无明显的影响。本研究在稻瘟菌中发现了一种新的真菌病毒,该病毒能够使稻瘟菌致病力下降,为稻瘟病的防治提供了潜在的病毒资源。
Rice blast is a worldwide disease caused by Magnaporthe grise. Each year, it causes a substantial reduction of rice output. Fungal viruses are viruses that can replicate and multiply in fungi and can be used as resources for biological control. In this experiment, the purified culture of Pyricularia grisea strain YC13 was obtained from the diseased rice leaves by single spore separation. The strain YC13 carries multiple dsRNA fragments, and the fragment F1 has 99% homology with the amino acid sequences of Coat Protein (CP) and RNA-dependent RNA polymerase (RdRp) of Magnaporthe oryzae virus 2 (MoV2) The sequences of F4 and F5 were 69% and 63% homologous to the RdRp and CP of Penicillium stoloniferum virus F (Ps VF) respectively, and named as Magnaporthe oryzae partitivirus virus 2 (MoPV2). The genome of MoPV2 contains two dsRNAs: dsRNA 1 encodes RdRp and dsRNA 2 encodes CP. According to the amino acid sequence of RdRp encoded by MoPV2, MoPV2 belongs to the genus Gammapartitivirus of the family Partitiviridae, which is a new fungal virus. MoPV2 was transferred to Magnaporthe grisea strain RB11 by viral particle transfection. Compared with strain RB11, the conidia production of RB11T25 was significantly reduced, and the pathogenicity of the RB11T25 strain was also decreased. However, other biological characteristics had no significant effect. In this study, a new fungal virus was found in Magnaporthe grisea. The virus can reduce the pathogenicity of Magnaporthe grisea and provide a potential viral resource for the control of blast.