论文部分内容阅读
以番茄Solanum lycopersicum为寄主植物,在pH 3.7、pH 4.5、pH 5.5和pH 6.5条件下接种根内根孢囊霉Rhizophagus intraradices,分别在培养4周和7周取样测定低pH对丛枝菌根真菌(AMF)丛枝发育和磷吸收利用的影响。结果表明,当pH低于5.5时,低pH显著抑制AMF对根系的侵染和丛枝的形成,且抑制效应随pH的降低而增强;与侵染率相比,丛枝丰度随土壤pH的降低而降低的幅度更大;低pH显著降低了植株生物量;与不接种处理相比,接种AMF显著提高植株生物量;相关分析表明,在菌根侵染指标中丛枝丰度与植株生长相关性最高;方差分解分析表明,pH对植株生物量的贡献率(88%和77%,两次取样)大于AMF的贡献率(5%和8%,两次取样);低pH对碱性磷酸酶活性的影响与根系侵染有相似的趋势;AMF能显著提高地上部P浓度,而低pH显著降低地上部P浓度以及根系中LePT3、LePT4和LePT5的表达。这些结果表明,低pH对AMF与植物的共生关系有显著的抑制作用,其中对丛枝的形成与功能的抑制效应最大。
Rhizophagus intraradices were inoculated with Solanum lycopersicum at pH 3.7, pH 4.5, pH 5.5 and pH 6.5, and the effects of low pH on arbuscular mycorrhizal fungi (AMF) on the development and the absorption and utilization of phosphorus. The results showed that when the pH was lower than 5.5, the low pH significantly inhibited the AMF infection on the roots and the formation of branches, and the inhibitory effect increased with the decrease of pH. Compared with the infection rate, the abundance of the branches increased with soil pH Decreased significantly, while low pH decreased plant biomass significantly. Compared with non-inoculation treatment, AMF significantly increased plant biomass. Correlation analysis showed that in the mycorrhizal colonization index, The variance analysis showed that the contribution of pH to plant biomass (88% and 77%, twice sampling) was greater than that of AMF (5% and 8%, twice sampling) The activities of phosphatase were similar to those of root inoculation. AMF significantly increased the P concentration in shoots, while the low pH significantly decreased the P concentration in shoots and the expression of LePT3, LePT4 and LePT5 in roots. These results indicate that low pH has a significant inhibitory effect on the symbiotic relationship between AMF and plants, with the greatest inhibitory effect on the formation and function of the clumps.