论文部分内容阅读
本文首次报告了应用过氧化物酶标记金葡菌 A 蛋白取代酶标第二抗体进行酶联免疫吸附试验(PPA—ELISA)检测实验家兔弓形体抗体的结果。24只感染兔和32只正常兔的阳性和阴性符合率均为100%,与16只爱美尔球虫病兔之间亦无交叉反应现象。采用滤纸血片标本检测,阳性符合率为100%,阴性符合率为96.87%,与间接血凝试验(IHA)比较,前法在弓形体感染后第11天8/11(72.72%)的家兔呈现阳性反应,而后法尚未能检出,提示 PPA-ELISA 较 IHA 具有更高的敏感性。文章叙述了弓形体遣殖子的分离纯化和抗原制备过程,并对胰蛋白酶处理前后的速殖子所制备的可溶性抗原在 PPA-ELISA 中的使用效果进行了比较。
In this paper, the results of the PPA-ELISA assay for the detection of Toxoplasma gondii antigens in rabbits using peroxidase-labeled Staphylococcus aureus protein A secondary antibody were reported for the first time. The positive and negative coincidence rates of all 24 infected rabbits and 32 normal rabbits were 100%, and there was no cross-reaction with 16 ehrlichosis rabbits. The positive coincidence rate was 100% and the negative coincidence rate was 96.87% when using the filter paper specimen. Compared with the indirect hemagglutination test (IHA), the former method was effective on the 11th day after Toxoplasma infection (8/11 (72.72%)) Rabbit showed a positive reaction, but after the law has not been detected, suggesting that PPA-ELISA than IHA has a higher sensitivity. The article described the isolation and purification of Toxoplasma gondii and the process of antigen preparation. The effect of using soluble antigen prepared by tachyzoites before and after trypsin treatment in PPA-ELISA was compared.