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把14个小麦基因型(11个四倍体和3个六倍体)同1个玉米杂交种F1进行杂交(玉米杂交种F1用作父本)。试验为随机区组设计.3个重复。植株种植在可调控的温室条件下(日长16h.昼/夜温度为25℃/15℃)。为了提高胚成活率.用2.4-D(10mg/L)处理玉米授过粉24h的小麦穗。四倍体和六倍体的胚成活率为每100个授粉小花中成活2.09—26.76个胚.在所有的六倍体小麦基因型和11个四倍体小麦基因型中.有5个均获得了单倍体和双倍体植株.本试验的最重要之处是能够用四倍体小麦培育单倍体植株.其理由有二:第一.由于无效胚的形成和高比率的白化苗不能用于对四倍体小麦进行花粉培养.第二,到目前为止.尽管可以用四倍体小麦同玉米杂交产生胚.但不能产生单倍体植株.
Fourteen wheat genotypes (11 tetraploids and 3 hexaploids) were crossed with 1 maize hybrid F1 (maize hybrid F1 was used as a male parent). The trial is a randomized block design. 3 repetitions. Plants are grown under controlled greenhouse conditions (day 16h, day / night at 25 ° C / 15 ° C). In order to improve the survival rate of embryos. Corn was spiked with 2.4-D (10 mg / L) for wheat for 24 h. Tetraploid and hexaploid embryos survived 2.09-26.76 embryos per 100 pollinated florets. In all hexaploid and 11 tetraploid wheat genotypes. Five of them obtained haploid and diploid plants. The most important aspect of this experiment is the ability to cultivate haploid plants with tetraploid wheat. There are two reasons for this: first. Because of the formation of inefficient embryos and high rates of albino seedlings, they can not be used for pollen tetraploid wheat. Second, so far. Although embryos can be produced by crossing tetraploid wheat with corn. But can not produce haploid plants.