方法 1 1.浸泡和染色取100克种子,浸泡于加有0.15克锥虫兰的1,000毫升5％的氢氧化钠溶液中,在22—24℃中染色22小时。 2.提取将样品转移到线虫分离器。(Fenwick容器),用软管连接热的自来水管。用热水流(60—65℃)搅动麦粒,使被分离的胚漂浮到容器顶部并随同水流通过容器边缘流入三个分级系列筛中,筛子按孔径3.5,2.0和1毫米递减依次安排。胚被收集 Method 1 1. Soaking and Dyeing 100 g of seeds are dipped in 1,000 ml of 5% sodium hydroxide solution plus 0.15 g of trypanosomes and stained for 22 hours at 22-24 ° C. 2. Extraction Transfer sample to nematode separator. (Fenwick container), hose to connect the hot water pipe. The kernels were agitated with a hot water flow (60-65 ° C) and the separated embryos were floated to the top of the container and flowed along the water flow through the edge of the container into three graded series of screens arranged in decreasing order of 3.5, 2.0 and 1 mm apertures. The embryos are collected