中药复方血清对HUVEC血管形成及Gli1 VEGF表达的影响

来源 :四川中医 | 被引量 : 0次 | 上传用户:kwl9970024
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的:观察中药复方肠复康(CFK)动物血清对HUVEC血管形成及Gli 1、VEGF表达的影响。方法:采用双室联合培养人脐静脉内皮细胞(HUVEC)和人结肠癌(Lovo)细胞,观察CFK含药血清对HUVEC血管形成的影响,并观察Gli 1及VEGF表达的变化。结果:CFK含药血清作用后,HUVEC增殖活性明显降低,其成管指数明显降低,Gli 1、VEGF表达明显减少,与对照组比较均有显著性差异(P<0.05或P<0.01)。结论:CFK具有抑制大肠癌血管生成作用。其机制与其抑制Hedgehog-Gli1信号通路激活,下调VEGF表达而发挥抗肿瘤血管形成作用有关。 Objective: To observe the effects of serum of traditional Chinese medicine compound Changfukang (CFK) on angiogenesis and the expression of Gli 1 and VEGF in HUVECs. Methods: Human umbilical vein endothelial cells (HUVECs) and human colon cancer cells (Lovo) were co-cultured in vitro. The effects of CFK-containing serum on the formation of HUVEC were observed. The changes of Gli 1 and VEGF were observed. Results: The proliferation of HUVEC significantly decreased after treated with CFK. The tube formation index was significantly decreased, while the expression of Gli 1 and VEGF was significantly decreased (P <0.05 or P <0.01). Conclusion: CFK can inhibit the angiogenesis of colorectal cancer. Its mechanism and its inhibition of Hedgehog-Gli1 signaling pathway activation, downregulation of VEGF expression and exert anti-tumor angiogenesis.
其他文献
目的:观察自拟益气解毒祛瘀汤治疗糖尿病足的疗效.方法:65例糖尿病足患者随机分为治疗组33例和对照组32例.2组均给予糖尿病足基础治疗,治疗组加用自拟益气解毒祛瘀汤(黄芪、
期刊
@@
溃疡性结肠炎发病符合伏邪致病特点,而透法是伏邪治疗的重要方法.风药因其辛散而常被用作“透药”,且有“宣透”、“渗透”、“通透”及“润透”之分.临床应用透法需注重药宜
期刊
@@
目的:观察针灸结合梅特兰手法治疗腰椎间盘突出症的临床疗效。方法:从绵阳市中医医院康复科选出符合纳入标准的156例腰椎间盘突出症血瘀证患者,按照随机对照原则,分别为治疗组
鸡西矿业集团公司张辰煤矿西三采区3
期刊
An 1.5D equilibrium evolution code was used to model the time evolution of thefirst ohmic discharges in the EAST experiment.Good agreement between the sinmlati
A new concept of a coaxial plasma dynamic accelerator with a self-energized mag-netic compressor coil to simulate the effects of space debris impact is demonst
1、中国开启新一轮全面深化改革事件:2013年11月9日~12日,中国共产党十八届三中全会召开,开启中国新一轮全面深化改革。会议通过了《中共中央关于全面深化改革若干重大问题的
目的:观察电针头针配合吞咽训练治疗脑卒中后假性球麻痹吞咽障碍疗效。方法:将80例脑卒中后假性球麻痹吞咽障碍患者,随机分为治疗组和对照组,每组40例,治疗组采用电针、头针
A Fokker-Planck code is developed based upon Epperlein’s scheme to investigatelaser-produced plasmas in relevance to inertial confinement fusion.The equations
如果说20世纪的意甲是繁花似锦的夏季,那么21世纪的意甲更像是落叶纷飞的秋天。在意甲强弩之末的那几年,依然为世界足坛贡献了不少现象级的球员,其中包括舍瓦、卡卡、伊布这