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目的评价Survivin基因表达对宫颈上皮内瘤变(CIN)Ⅰ转归的预测价值。方法选取天津市大港区人群子宫颈癌筛查项目中发现的未经治疗且具有预后随访资料的CINⅠ患者54例为研究对象,选其存档蜡块采用免疫组化二步法检测Survivin基因的表达,用正常宫颈组织20例作对照;评价Survivin基因表达与CINⅠ消退、持续及进展的关系。结果 54例CINⅠ中病变消退32例,病变持续19例,病变进展为CINⅡ与CINⅢ者共3例。Survivin基因在正常宫颈组织呈阴性表达。在病变消退、持续和进展的C INⅠ中的阳性表达率分别为59.38%,95.45%;表达强度分别为(0.59±0.50)分和(1.05±0.38)分。Survivin基因的阳性表达率在病变持续及进展与病变消退的C INⅠ中的差异有统计学意义(P<0.01)。结论 Survivin基因的阳性表达对CINⅠ病变的持续或进展具有阳性预测价值。Survivin基因的阴性表达对CINⅠ病变的消退具有一定的预测价值。
Objective To evaluate the predictive value of Survivin gene expression in the prognosis of cervical intraepithelial neoplasia (CIN) Ⅰ. Methods Totally 54 untreated CIN Ⅰ patients with cervical cancer screening in Dagang district of Tianjin were enrolled in this study. Survivin gene expression was detected by immunohistochemical two-step method , 20 cases of normal cervical tissue as a control; evaluation of Survivin gene expression and CIN Ⅰ regression, the relationship between the duration and progress. Results Of the 54 patients with CINⅠ, 32 patients had subsided, 19 patients had lesions, and 3 patients had CINⅡ and CINⅢ. Survivin gene was negative in normal cervical tissue. The positive expression rates of C IN Ⅰ in the subsided, persistent and progressive lesions were 59.38% and 95.45%, respectively; the expression intensity was (0.59 ± 0.50) and (1.05 ± 0.38) points respectively. The positive expression rate of Survivin gene was significantly different between CINⅠand CINⅠ (P <0.01). Conclusion The positive expression of Survivin gene has a positive predictive value for the duration or progression of CIN Ⅰ. The negative expression of Survivin gene has some predictive value for the regression of CIN Ⅰ disease.