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目的 了解妊娠高血压综合征 (妊高征 )胎盘组织基因表达的改变 ,确定与妊高征发病相关的基因 ,为研究妊高征的发病机理提供线索。 方法 收集 2 0 0 1年 3月至 2 0 0 2年 9月西安地区以剖宫产终止妊娠的 4 2例妊高征患者及 2 2例正常产妇的产前、产后临床资料 ;收集胎盘组织 ,制作胎盘组织芯片 ;建立妊高征患者数据库。采用抑制消减杂交技术 (suppressionsubtractivehybridi za tion ,SSH)建立妊高征胎盘组织cDNA消减文库 ;利用差异筛选技术筛选出妊高征胎盘高表达基因 ,用RT PCR和免疫组织化学技术验证部分高表达基因。 结果 建立了由 4 2例中、重度妊高征患者和 2 2例正常产妇组成的数据库 ;用 6 6例胎盘组织制成 15 0点的胎盘组织芯片。利用SSH和差异筛选共分离出 10 3个阳性克隆 ,测序成功 90个。测序结果显示 90个cDNA片断与GenBank/EMBL中36条已知功能基因有 95 %以上的同源性。 结论 妊高征患者胎盘组织基因表达发生较大变化 ;有必要研究血管内皮生长因子受体、卵巢抑制素、丝氨酸蛋白酶抑制素、细胞角蛋白 7以了解妊高征的发病机理。
Objective To understand the gene expression changes of placenta in pregnancy induced hypertension syndrome (PIH) and to identify the genes involved in the pathogenesis of PIH, so as to provide clues for studying the pathogenesis of PIH. Methods Prenatal and postnatal clinical data of 42 cases of pregnancy induced hypertension induced by cesarean section in Xi’an from March 2001 to September 2002 were collected. Prenatal and postnatal clinical data were collected. Placenta tissue , Making placenta tissue chip; establishing a database of patients with pregnancy-induced hypertension. The cDNA subtractive library of placenta of PIH was established by suppressionsubtractivehybridization (SSH). Highly expressed placental genes of PIH were screened by differential screening, and some highly expressed genes were verified by RT-PCR and immunohistochemistry. Results A database of 42 pregnant women with severe PIH and 22 normal pregnant women was established. Six hundred and sixty placenta tissues were used to make 150 placenta tissue chips. A total of 10 3 positive clones were isolated by SSH and differential screening, and 90 were sequenced successfully. Sequencing results showed that 90 cDNA fragments shared 95% homology with 36 known functional genes in GenBank / EMBL. Conclusion The gene expression of placenta in patients with PIH is greatly changed. It is necessary to study the pathogenesis of PIH by investigating the expression of vascular endothelial growth factor receptor, ovarian inhibin, serpin and cytokeratin 7.