论文部分内容阅读
目的:探讨人参皂苷Rg1对高血糖所致心肌损害的保护作用及其机制。创新点:使用糖尿病大鼠为实验对象,探讨三种浓度的人参皂苷Rg1对糖尿病心肌损伤的保护作用及其机制,检测其是否具有浓度依赖性。方法:将60只Wistar大鼠随机分组,其中空白对照组10只,另50只给予高脂高糖饲养,4周后腹腔注射40 mg/kg链脲佐菌素(STZ)。成功制备糖尿病大鼠模型40只,再随机分为糖尿病模型组,糖尿病大鼠+低剂量人参皂苷Rg1(10 mg/(kg·d)),糖尿病大鼠+中剂量人参皂苷Rg1(15 mg/(kg·d)),糖尿病大鼠+高剂量人参皂苷Rg1(20 mg/(kg·d))。12周后处死大鼠,取血测定空腹血糖、总胆固醇(TC)、甘油三酯(TG)、心肌酶及氧化应激水平,留取心肌组织使用透射电镜观察心肌细胞超微结构改变,应用TUNEL法检测心肌细胞凋亡,免疫组化检测细胞凋亡相关蛋白半胱氨酸天冬氨酸蛋白酶3(CASP3)和Bcl-x L的表达。结论:人参皂苷Rg1对糖尿病大鼠糖脂代谢无明显影响,人参皂苷Rg1可降低糖尿病大鼠血清肌钙蛋白(c Tn I)和肌酸激酶同工酶(CK-MB)水平,改善心肌细胞超微结构,减少心肌细胞凋亡,降低大鼠血清和心肌组织中丙二醛(MDA)含量,提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH)水平,降低凋亡蛋白CASP3的表达,同时提高Bcl-x L蛋白表达。总之,人参皂苷Rg1能显著保护糖尿病大鼠心肌损伤,其机制可能与其抗氧化及抗细胞凋亡作用有关。
Objective: To investigate the protective effect of ginsenoside Rg1 on myocardial damage induced by hyperglycemia and its mechanism. Innovative point: Diabetic rats were used as experimental subjects to explore the protective effects of three concentrations of ginsenoside Rg1 on diabetic myocardial injury and its mechanism, to determine whether it has a concentration-dependent manner. Methods: Sixty Wistar rats were randomly divided into control group (n = 10) and high fat and high glucose (n = 50). Four weeks later, streptozotocin (STZ) was injected intraperitoneally. Forty diabetic rats were successfully prepared and then randomly divided into diabetic model group, diabetic rats + low dose ginsenoside Rg1 (10 mg / (kg · d)), diabetic rats + medium dose ginsenoside Rg1 (15 mg / (kg · d)), diabetic rats + high dose of ginsenoside Rg1 (20 mg / (kg · d)). After 12 weeks, the rats were sacrificed and the fasting blood glucose, total cholesterol (TC), triglyceride (TG), myocardial enzymes and oxidative stress were measured. The ultrastructure of myocardium was observed by transmission electron microscopy. Cardiomyocyte apoptosis was detected by TUNEL method. The expressions of caspase 3 (CASP3) and Bcl-x L were detected by immunohistochemistry. CONCLUSION: Ginsenoside Rg1 has no significant effect on the glucose and lipid metabolism in diabetic rats. Ginsenoside Rg1 can reduce the levels of cTn I and CK-MB in diabetic rats and improve the cardiomyocyte Ultrastructure, reduce the cardiomyocyte apoptosis, reduce the content of malondialdehyde (MDA) in the serum and myocardial tissue, increase the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidation (GSH) levels, reducing the expression of apoptotic protein CASP3, while increasing the expression of Bcl-x L protein. In conclusion, ginsenoside Rg1 can significantly protect myocardium from diabetic rats, and its mechanism may be related to its anti-oxidation and anti-apoptotic effects.