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细胞与细胞融合在1型人免疫缺陷病毒(HIV-1)感染的发病机理中起着重要作用。作者研制了一种测定血清中抗融合活性的方法,并根据中性红摄入与细胞培养物中合体细胞减少相关联的证据,评价了血清中的抗融合活性。本法用96孔微量稀释板操作。所有血清在用前均于56℃下加热60分钟灭活。每次测定时用HIV-1血清学阴性者血清和阳性者标准血清作对照。除一排用作空白对照外,各孔均加50μl生长培养液。在底排3～11孔加50μl试验血清并做1:2连续稀释,一式三
Cell-cell fusion plays an important role in the pathogenesis of human immunodeficiency virus type 1 (HIV-1) infection. The authors developed a method for the determination of anti-fusion activity in serum and evaluated the anti-fusion activity in serum based on the evidence that neutral red uptake correlates with syncytial cell depletion in cell cultures. This method with 96-well micro-dilution plate operation. All sera were inactivated by heating at 56 ° C for 60 minutes before use. HIV-1 seronegative serum and positive human serum were used as controls for each assay. Except for a row served as a blank control, 50 μl of growth broth was added to each well. In the bottom row 3 to 11 wells plus 50μl of test serum and do 1: 2 serial dilution, in triplicate