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目的探讨结肠癌患者N末端截短型p63基因(ΔNp63)和核转录因子κB(NF-κB)的表达及意义。方法采用免疫组织化学法检测结肠癌患者ΔNp63和NF-κB的表达;采用实时荧光定量聚合酶链反应(q RT-PCR)和Western blot检测ΔNp63和NF-κB信使核糖核酸(m RNA)和蛋白的表达;采用半胱氨酸的天冬氨酸蛋白水解酶3(Caspase-3)活性检测试剂盒检测凋亡蛋白Caspase-3表达。结果与对照组比较,结肠癌患者ΔNp63蛋白表达升高35.57%,NF-κB蛋白表达升高58.93%,差异有统计学意义(P<0.05);结肠癌患者ΔNp63和NF-κB m RNA和蛋白表达均升高,差异有统计学意义(P<0.05);结肠癌患者Caspase-3活性升高,差异有统计学意义(P<0.05)。结论结肠癌患者ΔNp63和NF-κB呈过度表达状态;ΔNp63和NF-κB的过表达在结肠癌发生、发展机制中可能起到重要作用。
Objective To investigate the expression and significance of N-terminal truncated p63 gene (ΔNp63) and nuclear factor-κB (NF-κB) in patients with colon cancer. Methods Immunohistochemistry was used to detect the expression of ΔNp63 and NF-κB in colon cancer patients. △Np63 and NF-κB messenger RNA (m RNA) and protein were detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) and Western blot. The expression of apoptotic protein Caspase-3 was detected using cysteine-aspartate proteinase 3 activity assay kit. Results Compared with the control group, the expression of ΔNp63 protein was increased by 35.57% and the expression of NF-κB protein was increased by 58.93% in colon cancer patients (P<0.05); ΔNp63 and NF-κB m RNA and protein in colon cancer patients The expression was increased and the difference was statistically significant (P<0.05). The activity of Caspase-3 was increased in colon cancer patients, and the difference was statistically significant (P<0.05). Conclusion ΔNp63 and NF-κB are overexpressed in patients with colon cancer. The overexpression of ΔNp63 and NF-κB may play an important role in the occurrence and development of colon cancer.