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目的:建立近红外漫反射光谱法测定甘西鼠尾草药材及总酚酸提取物中迷迭香酸和丹酚酸B的含量。方法:设立校正样品集与测试样品集,采集近红外光谱数据,运用偏最小二乘法建立数学模型,对测试样品样品集进行预测。结果:迷迭香酸:校正样品集的最佳主成分数Rank=12,内部交叉验证均方差RMSECV=0.129、决定系数R~2=99.95。丹酚酸B:校正样品集的最佳主成分数Rank=9,内部交叉验证均方差RMSECV=0.426、决定系数R~2=99.26。结论:采用近红外漫反射光谱法对甘西鼠尾草药材及总酚酸提取物进行质量控制,方法简明、方便快捷、数据准确,适用于大量、快速、方便的药材质量鉴定。
Objective: To establish a method for the determination of rosmarinic acid and salvianolic acid B in Salvia miltiorrhiza and total phenolic acids extract by near infrared diffuse reflectance spectroscopy. Methods: A calibration sample set and a test sample set were set up to collect near-infrared spectroscopy data. Partial least-squares method was used to establish a mathematical model to predict the test sample set. Results: Rosmarinic acid: the best principal component of calibration sample set Rank = 12, the internal cross validation RMSECV = 0.129, the determination coefficient R ~ 2 = 99.95. Salvianolic acid B: The optimal principal component Rank of the calibration sample set = 9, the internal cross-validation mean square error RMSECV = 0.426, the determination coefficient R ~ 2 = 99.26. Conclusion: Near-infrared diffuse reflectance spectroscopy is used to control the quality of Salvia miltiorrhiza Bunge. And its total phenolic acids extract. The method is simple, convenient, and accurate. It is suitable for mass, rapid and convenient identification of medicinal materials.