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目的研究山茱萸生、制品二氯甲烷萃取部位对D-半乳糖致衰小鼠骨髓细胞的影响。方法单细胞电泳观察D-半乳糖致衰小鼠骨髓细胞DNA损伤情况,RT-PCR法检测小鼠骨髓细胞p53基因的表达情况。结果D-半乳糖致衰模型组小鼠骨髓细胞DNA拖尾率高于正常对照组(P<0.05),且Ⅲ、Ⅳ级(中重度)损伤细胞数较多;山茱萸生品及制品二氯甲烷萃取部位组小鼠骨髓细胞DNA的拖尾率均低于D-半乳糖致衰模型组(P<0.05)。D-半乳糖致衰模型组小鼠骨髓细胞p53基因的表达高于正常对照组、阳性药组及制品组。结论D-半乳糖致衰小鼠骨髓细胞DNA有损伤,山茱萸生、制品二氯甲烷萃取部位对D-半乳糖致衰小鼠骨髓细胞有保护作用;D-半乳糖致衰小鼠骨髓p53基因表达上调,山茱萸制品二氯甲烷萃取部位能显著下调D-半乳糖致衰小鼠骨髓p53基因的表达。
Objective To study the effects of extracts of methylene chloride extracts and products on bone marrow cells in D-galactose-induced mice. Methods Single cell electrophoresis was used to observe the DNA damage of bone marrow cells in D-galactose-induced mice, and the expression of p53 gene in bone marrow cells was detected by RT-PCR. Results The DNA tailing rate of bone marrow cells in the D-galactose-induced model group was higher than that in the normal control group (P<0.05), and the number of damaged cells in grades III and IV (moderately severe) was higher; Hawthorn raw products and products were dichlorine The tailing rate of DNA in mouse bone marrow cells in the methane extract group was lower than that in the D-galactose degeneration model group (P<0.05). The expression of p53 gene in bone marrow cells of D-galactose-induced model group was higher than that of normal control group, positive drug group and product group. Conclusion The DNA damage of bone marrow cells in D-galactose-induced mice was impaired, and the extract of methylene chloride from hawthorn and product had protective effect on bone marrow cells in D-galactose-induced mice. The bone marrow p53 gene was induced in D-galactose-induced mice. Expression was up-regulated. The methylene chloride extract from hawthorn products could significantly down-regulate the expression of p53 gene in bone marrow of D-galactose-induced mice.