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目的：研究糖基化终产物（ＡＧＥＰ）对主动脉平滑肌细胞增殖的影响及其与［Ｃａ２＋］ｉ的关系．方法：采用同位素掺入法分别测定ＤＮＡ和蛋白质合成；Ｆｕｒａ２ＡＭ测定［Ｃａ２＋］ｉ．结果：ＡＧＥＰ以浓度、时间相关的方式促进［３Ｈ］ＴｄＲ与［３Ｈ］Ｌｅｕ掺入细胞，随ＡＧＥＰ作用时间、糖化时间延长，掺入率增加明显．ＡＧＥＰ增加［Ｃａ２＋］ｉ，与时间、浓度相关，但随ＡＧＥＰ作用时间延长（４０分钟后）而有所降低，ＢＳＡ修饰中葡萄糖浓度的增加，糖基化时间延长，［Ｃａ２＋］ｉ也呈上升趋势．结论：ＡＧＥＰ刺激平滑肌细胞增殖，并与细胞［Ｃａ２＋］ｉ浓度增加有关．
AIM: To investigate the effect of advanced glycation end products (AGEP) on the proliferation of aortic smooth muscle cells and its relationship with [Ca2 +] i. Methods: DNA and protein synthesis were determined by isotope incorporation method. Fura2AM was used to determine [Ca2 +] i. Results: AGEP promoted the incorporation of [3H] TdR and [3H] Leu into cells in a concentration - and time - dependent manner. With AGEP acting time and saccharification time increasing, the incorporation rate increased significantly. AGEP increased [Ca2 +] i, which was related to time and concentration, but decreased with the prolongation of AGEP (after 40 minutes). Increased glucose concentration, prolonged glycosylation time and [Ca2 +] i increased in BSA modification trend. Conclusion: AGEP stimulates the proliferation of smooth muscle cells and is related to the increase of [Ca2 +] i concentration in cells.