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目的探讨cyclin L2基因在化疗药物顺铂(DDP)、5-氟尿嘧啶(5-FU)和多西紫杉醇(Doc)诱导的肝癌细胞凋亡中的作用。方法用肝癌SMMC7721细胞株进行培养传代,MTT试验检测DDP、5-FU和Doc不同药物浓度对肝癌细胞生长的抑制作用,以及cyclin L2转染及反义转染后对该细胞生长抑制作用的影响。用流式细胞术定量检测细胞凋亡。结果不同浓度DDP、5-FU和Doc对肝癌细胞生长均有明显的浓度依赖性抑制作用。细胞凋亡率和cyclin L2基因表达率成正相关。在5-FU和DDP作用下,cyclin L2转染组凋亡率均高于对照组(P<0·01),反义转染组细胞凋亡率低于对照组(P>0·05)。结论Cyclin L2基因在化疗药物诱导肝癌细胞凋亡中起重要作用。
Objective To investigate the role of cyclin L2 gene in the apoptosis of hepatocellular carcinoma cells induced by cisplatin (DDP), 5-fluorouracil (5-FU) and docetaxel (Doc). Methods Hepatoma SMMC7721 cells were cultured and passaged. MTT assay was used to detect the inhibitory effect of different concentrations of DDP, 5-FU and Doc on the growth of hepatoma cells, and the effect of cyclin L2 transfection and antisense transfection on the growth inhibition of hepatoma cells. . Apoptosis was quantified by flow cytometry. Results Different concentration of DDP, 5-FU and Doc had a significant concentration-dependent inhibitory effect on the growth of hepatoma cells. The rate of apoptosis was positively correlated with the rate of cyclin L2 expression. Under the action of 5-FU and DDP, the apoptosis rate of cyclin L2 transfected group was higher than that of the control group (P<0.01), and the apoptosis rate of antisense transfection group was lower than that of the control group (P>0.05). . Conclusion The Cyclin L2 gene plays an important role in the apoptosis of hepatoma cells induced by chemotherapeutic drugs.