论文部分内容阅读
目的 :探讨肠病毒 RNA和抗 ANT抗体在健康献血员中检出的临床意义。方法 :应用酶联免疫吸附实验 (EL ISA)和逆转录 -聚合酶链式反应 (RT- PCR)方法对健康献血员和扩张型心肌病 (DCM)患者进行抗心肌多肽自身抗体 (抗 ANT抗体 )和肠病毒 (RNA)的检测。结果 :48例健康献血员中有 4例抗 ANT抗体阳性 (阳性率为 8.3% ) ,3例肠病毒 RNA阳性 (阳性率为 6 .3% ) ,而 48例 DCM患者中有 31例抗 ANT抗体阳性 (阳性率为6 4.6 % ) ,2 9例肠病毒 RNA阳性 (阳性率为 6 0 .4% ) ,两组比较差异有显著性意义 (P <0 .0 5 )。在 DCM组中 ,自身抗体和肠病毒 RNA的检出具有一致性 (r =0 .5 6 ) ,而健康献血员中 ,二者则没有相关性 (r =0 .0 8)。结论 :健康人可能存在肠病毒隐性感染 ,但不一定能激活自身免疫系统引起自身免疫损伤而导致 DCM;而肠病毒持续感染与肠病毒感染后激活自身免疫系统引起自身免疫损伤则可能是 DCM的主要发病机制。
Objective: To investigate the clinical significance of enterovirus RNA and anti-ANT antibody detected in healthy blood donors. Methods: The anti-myocardial anti-ANT antibody (anti-ANT antibody) was detected in patients with healthy blood donors and DCM by ELISA and RT-PCR. ) And enterovirus (RNA). RESULTS: Four out of 48 healthy blood donors were positive for anti-ANT antibody (positive rate was 8.3%), three were positive for enterovirus RNA (positive rate was 6.3%), whereas 31 of 48 DCM patients were anti-ANT (Positive rate was 6 4.6%), 29 cases of enterovirus RNA positive (positive rate was 60.4%), the difference between the two groups was significant (P <0. 05). In the DCM group, autoantibodies and enterovirus RNA were detected consistently (r = 0.56), but healthy blood donors showed no correlation (r = 0. 08). CONCLUSIONS: Infectious bowel virus may exist in healthy individuals, but may not activate the autoimmune system to cause autoimmune damage and lead to DCM. However, autoimmune damage caused by enterovirus infection and subsequent activation of the autoimmune system may be caused by DCM The main pathogenesis.