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目的：探讨氧自由基在肝缺血再灌注损伤（ＨＩＲＩ）中的作用及川芎嗪的防护作用。方法：应用ＨＩＲＩ家兔及肝癌手术患者，观察超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨ－ＰＸ），黄嘌呤氧化酶（ＸＯ）、谷丙转氨酶（ＡＬＴ）活性、丙二醛（ＭＤＡ）含量、肝细胞形态学变化及川芎嗪对上述指标的影响。结果：ＨＩＲＩ时ＳＯＤ、ＧＳＨ－ＰＸ活性显著下降（Ｐ＜０．０５和Ｐ＜０．０１），ＸＯ、ＡＬＴ活性及ＭＤＡ含量明显升高（Ｐ＜０．０５和Ｐ＜０．０１），肝细胞形态学发生异常变化；使用川芎嗪后，上述指标的异常变化均明显减轻，其差异有显著和非常显著（Ｐ＜０．０５和Ｐ＜０．０１）。结论：氧自由基是ＨＩＲＩ的重要发病学因素，而川芎嗪通过清除氧自由基可减轻ＨＩＲＩ。
Objective: To investigate the role of oxygen free radicals in hepatic ischemia-reperfusion injury (HIRI) and the protective effect of ligustrazine. Methods: HIRI rabbits and hepatocellular carcinoma patients were used to observe the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), xanthine oxidase (XO) and alanine aminotransferase , Malondialdehyde (MDA) content, morphological changes of liver cells and ligustrazine on the above indexes. Results: The activities of SOD and GSH-PX were significantly decreased (P <0.05 and P <0.01) and the activity of XO, ALT and the contents of MDA were significantly increased at HIRI (P <0.05 and P <0.01) The morphology of hepatocytes changed abnormally. The abnormal changes of the above indexes were obviously alleviated after using ligustrazine, the difference was significant and very significant (P <0.05 and P <0.01). Conclusion: Oxygen free radicals are the important pathogenetic factors of HIRI, and ligustrazine can reduce HIRI by scavenging oxygen free radicals.