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目的观察基质细胞衍生因子-1α(SDF-1α)对体外培养的大鼠骨髓源性内皮祖细胞(EPCs)动员和增殖的影响。方法微孔法获取大鼠骨髓EPCs并采用荧光显微镜和流式细胞术鉴定EPCs特异性标记物。不同浓度SDF-1α处理EPCs后,采用细胞培养、MTT检测EPCs的克隆形成、细胞增殖。结果通过MTT法检测,对照组与(1、10、100μg/L)SDF-1α处理组的OD值分别为0.311±0.054、0.587±0.072、0.813±0.056、1.029±0.078,通过统计学方法分析,对照组与SDF-1α处理组比较差异均有统计学意义(n=5,P<0.05);100μg/LSDF-1α处理组次级内皮祖细胞集落单位数目是对照组近3倍(4.67±1.577比14.33±3.055,n=5,P<0.01)。结论SDF-1α剂量依赖性地促进EPCs增殖,并显著增强EPCs克隆形成能力。
Objective To observe the effect of SDF-1α on mobilization and proliferation of rat bone marrow-derived endothelial progenitor cells (EPCs) cultured in vitro. Methods EPCs of rat bone marrow were obtained by micropore method. EPCs specific markers were identified by fluorescence microscopy and flow cytometry. After EPCs were treated with different concentrations of SDF-1α, cell culture and MTT were used to detect the formation of EPCs and cell proliferation. Results The MTT assay showed that the OD values of SDF-1α treated group and control group were 0.311 ± 0.054, 0.587 ± 0.072, 0.813 ± 0.056 and 1.029 ± 0.078, respectively. By statistical analysis, There was significant difference between the control group and the SDF-1α group (n = 5, P <0.05). The number of the secondary EPCs in the 100 μg / LSDF-1α group was nearly three times of that of the control group (4.67 ± 1.577 Than 14.33 ± 3.055, n = 5, P <0.01). Conclusion SDF-1α can promote the proliferation of EPCs dose-dependently and significantly enhance the clonality of EPCs.