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目的观察清毒汤对实验性重症肝损伤大鼠内毒素(endotoxin,ET)、肿瘤坏死因子α(tumor necrosis factor alpha,TNF-α)以及核转录因子κB(nuclear factor kappa B,NF-κB)表达的影响,探讨该方治疗慢性重型肝炎内毒素血症的作用机制。方法建立硫代乙酰胺(thioacetamide,TAA)致实验性大鼠重症肝损伤模型,给予清毒汤干预后,检测大鼠ET、TNF-α以及NF-κB表达的水平。结果与正常组比较,模型组大鼠血清ET、TNF-α以及NF-κB蛋白含量水平均显著升高(P<0.01);与模型组比较,清毒汤中、低剂量组和乳果糖组ET、TNF-α以及NF-κB蛋白含量水平显著降低(P<0.01),清毒汤高剂量组除ET外各指标均明显改善(P<0.05);与乳果糖组比较,清毒汤中剂量组ET及TNF-α水平显著降低(P<0.01),低剂量组ET及TNF-α水平无明显差异(P>0.05),高剂量组ET及TNF-α水平明显高于乳果糖组(P<0.05)。结论清毒汤对实验性肝损伤大鼠模型具有降低ET、TNF-α以及NF-κB蛋白水平的作用,尤其中剂量组效果最为明显,推测其分子机制可能为通过抑制NF-κB因子的表达从而达到保肝目的。
Objective To observe the effects of qingdu decoction on endotoxin (ET), tumor necrosis factor alpha (TNF-α) and nuclear factor kappa B (NF-κB) in rats with severe experimental hepatic injury Expression of the impact of the side treatment of chronic severe hepatitis endotoxemia mechanism of action. Methods A rat model of severe liver injury induced by thioacetamide (TAA) was established. After the intervention of qingdu decoction, the levels of ET, TNF-α and NF-κB were detected. Results Compared with the normal group, the levels of serum ET, TNF-α and NF-κB in the model group were significantly increased (P <0.01). Compared with the model group, the middle and low dose Qinglintang group and lactulose group The levels of ET, TNF-α and NF-κB were significantly decreased (P <0.01). The indexes of high-dose Qingqintang group except ET were significantly improved (P <0.05). Compared with lactulose group, (P <0.01), ET and TNF-αlevels in low-dose group had no significant difference (P> 0.05), ET and TNF-αlevel in high dose group was significantly higher than that in lactulose group P <0.05). Conclusion Qingdu decoction can reduce ET, TNF-α and NF-κB protein levels in the rat model of hepatic injury, especially in the middle-dose group. It is presumed that the molecular mechanism may be through inhibiting the expression of NF-κB To achieve the purpose of liver protection.