论文部分内容阅读
目的:制备两性霉素B立方液晶前体胶囊,并考察体外释放度。方法:以单油酸甘油酯为液晶材料、两性霉素B为主药制备了立方液晶前体胶囊;用偏光显微镜及小角衍射表征其吸水后的相态;采用高效液相色谱法测定体外释放度。结果:立方液晶前体胶囊吸水后在偏光显微镜下呈暗背景;小角衍射峰位符合q1∶q2=槡6∶槡8,显示其内部结构为Ia3d(Q230)型立方液晶;立方液晶前体胶囊在含有0.25%十二烷基硫酸钠(SDS)的pH 7.4磷酸盐缓冲液(PBS 7.4溶液)中持续释药达9 h。结论:立方液晶前体胶囊在生理温度下接触水介质后可快速吸水形成立方液晶,并具有一定的缓释作用。
Objective: To prepare amphotericin B cubic liquid crystal precursor capsules and investigate the in vitro release. Methods: The liquid crystal material of monoolein was used as the liquid crystal material and amphotericin B was used as the main drug to prepare the cubic liquid crystal precursor capsules. The phase state after water absorption was characterized by polarized light microscopy and small-angle diffraction. The in vitro release was determined by high performance liquid chromatography degree. Results: The cubic liquid crystal precursor capsule showed a dark background under the light microscope after absorbing water. The small angle diffraction peak was in line with q1: q2 = 槡6: 槡8, indicating that its internal structure was cubic liquid crystal of Ia3d (Q230) Sustained release was achieved for 9 h in pH 7.4 phosphate buffer (PBS 7.4 solution) containing 0.25% sodium dodecyl sulfate (SDS). CONCLUSION: Cubic liquid crystal precursor capsules can quickly absorb water to form cubic liquid crystal when exposed to aqueous medium at physiological temperature, and have certain sustained release effect.