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针对溶剂萃取法获得的姜油树脂,用AB8大孔吸附树脂作为分离载体,乙醚和正己烷作为洗脱剂体系分离姜油树脂中的有效成分姜酚。用GC-MS对实验样品进行了表征,用峰面积归一化法确定了各组分的峰面积百分数,与姜酚标准样品对照,确定了姜酚的富集率。结果表明,当选用乙醚和正己烷组成洗脱剂体系时,经分离后的样品中成分种类减少,由姜油树脂中的7大类物质27种成分,减少到分离后的3大类物质11种成分;姜油树脂中占相当比例的烯类物质峰面积百分数明显下降,姜酚的峰面积百分数由姜油树脂原样中的1.27%提高到55.68%,分离效果明显,富集率达70.88%。
For the ginger oil resin obtained by solvent extraction, AB8 macroporous resin was used as the separation carrier, ether and n-hexane as the eluent system to separate ginger oil from the active ingredient gingerol. The experimental samples were characterized by GC-MS, and the peak area percentages of each component were determined by peak area normalization method. Compared with gingerol standard samples, the concentration of gingerol was determined. The results showed that when the eluent system was composed of diethyl ether and n-hexane, the content of the components in the separated sample decreased and the content of 27 components in the seven major categories of ginger oleoresin decreased to three categories of substances 11 The percentage of peak area of alkene which accounted for a considerable proportion of ginger oleoresin decreased significantly, the peak area percentage of gingerol increased from 1.27% to 55.68% in original sample of ginger oleoresin, and the separation effect was obvious with an enrichment rate of 70.88% .