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目的:探讨桂枝-生姜药对超临界CO2萃取物抗甲型H1N1流感病毒(2009流行株)的有效成分,以期诠释其药效物质基础。方法:采用超临界CO2萃取法提取桂枝-生姜药对的有效物质部位,通过细胞病变效应(CPE)与噻唑蓝(MTT)比色法对其抗甲型H1N1流感病毒感染狗肾细胞(MDCK)的作用进行比较研究,并用GC/MS技术分析鉴定其化学成分。结果:桂枝-生姜超临界CO2萃取物对甲型H1N1流感病毒具有一定抑制作用,且成明显的量-效关系,其半数抑制浓度(IC50)为63.6μg·mL-1,治疗指数(TI)为1.2,阳性对照药利巴韦林对流感病毒直接灭活作用的IC50为453.2μg·mL-1,TI为1.0;从桂枝-生姜药对的超临界CO2萃取物中分离鉴定出55个成分,占成分含量的97.29%,其桂皮醛占62.85%,2-甲氧基肉桂醛占16.03%,邻苯二甲酸二异辛酯占7.99%,主要为醛、酮、烯、酯类化合物。结论:桂枝-生姜药对超临界CO2萃取物具有一定的抗甲型H1N1流感病毒作用,作用形式为直接灭活,且成剂量依赖性。
Objective: To investigate the effective constituents of Guizhi-Ginger on the supercritical CO2 extract of anti-H1N1 influenza virus (2009 epidemic strain), in order to explain the material basis of its efficacy. Methods: The active substance of Guizhi - Ginger was extracted by supercritical CO2 extraction. The effects of anti-influenza A (H1N1) virus on canine kidney cells (MDCK) were detected by cytopathic effect (CPE) and thiazolyl tetrazolium ) Of the role of comparative studies, and GC / MS analysis of chemical components identified. Results: The extract of Guizhi-Ginger supercritical CO2 had certain inhibitory effect on influenza A (H1N1) virus in a dose-effect relationship. The IC50 was 63.6 μg · mL-1, and the therapeutic index (TI ) Was 1.2. The IC50 value of positive control ribavirin for direct inactivation of influenza virus was 453.2μg · mL-1, TI was 1.0. The supercritical CO2 extract of Guizhi-Ginger was isolated and identified as 55 Component, accounting for 97.29% of the component content, cinnamic aldehyde accounted for 62.85%, 2-methoxycinnamaldehyde accounted for 16.03%, dioctyl phthalate accounted for 7.99%, mainly aldehydes, ketones, alkenes, esters Compound. Conclusion: Guizhi-Ginger has certain anti-influenza A (H1N1) virus effect on supercritical CO2 extract. Its action form is direct inactivation and dose-dependent.