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[目的]筛选对苏云金芽孢杆菌(Bacillusthuringiensis,简称Bt)可湿性粉剂具有最佳增效作用的物质,并对增效作用显著的混配组合进行了增效作用的评定。[方法]以小菜蛾为生物测定昆虫,采用浸叶法测定了6种添加物对苏云金杆菌(Bacillusthuringiensis,Bt)可湿性粉剂的毒杀增效作用。[结果]研究发现0.1%ZnCl2、0.5%ZnCl2、1.0%ZnCl2、1.0%MgCl2、0.5%硼酸、1.0%硼酸、0.5%柠檬酸和1.0%柠檬酸对Bt可湿性粉剂表现出增效作用,其中0.5%硼酸对Bt可湿性粉剂的增效作用最强,增效比为17.2,1.0%ZnCl2次之,增效比为15.6。同时,杀虫速度的测定结果显示,0.5%硼酸的加入使Bt可湿性粉剂的致死中时缩短了约10h,加快了Bt的杀虫速度。此外,当将1.0%ZnCl2和0.5%硼酸分别与Bt可湿性粉剂溶液室温下混合放置15d后,两种混合物的毒力均没有明显的变化。[结论]硼酸作为Bt可湿性粉剂的增效因子,不但有利于增强Bt制剂的杀虫效果而且还可以提高其杀虫速度,从而在一定程度上克服了Bt制剂杀虫效果差和杀虫速度慢等实际问题。
[Objective] The research aimed to select the best synergistic substances for Bacillus thuringiensis (Bt) WP and evaluate the synergistic effect of the synergistic combination. [Method] The insecticidal activity of six kinds of additives to Bacillus thuringiensis (Bt) wettable powder was determined by dipping leaf method with diamondback moth as a bioassay insect. [Result] The results showed that 0.1% ZnCl2, 0.5% ZnCl2, 1.0% ZnCl2, 1.0% MgCl2, 0.5% boric acid, 1.0% boric acid, 0.5% citric acid and 1.0% citric acid showed synergistic effect on Bt WP, 0.5% boric acid has the strongest synergistic effect on Bt WP, with a synergistic ratio of 17.2 and 1.0% ZnCl2, with a synergistic ratio of 15.6. Meanwhile, the determination of insecticidal speed showed that the addition of 0.5% boric acid shortened the lethal time of Bt WP by about 10h, accelerating the insecticidal rate of Bt. In addition, when the mixture of 1.0% ZnCl2 and 0.5% boric acid were mixed with Bt WP solution at room temperature for 15 days respectively, there was no obvious change in the virulence of the two mixtures. [Conclusion] Boric acid, as a synergistic factor of Bt WP, not only can enhance the insecticidal effect of Bt formulation but also increase the insecticidal rate of Bt formulation, which can overcome the insecticidal effect and insecticidal speed of Bt formulation to a certain extent Slow and other practical problems.