论文部分内容阅读
目的观察人芽囊原虫(Blastocystis hominis)感染小鼠肠道组织中白细胞介素17(IL-17)和IL-23的表达情况。方法30只BABL/c小鼠随机分为实验组、免疫抑制剂组和对照组,实验组和免疫抑制剂组小鼠腹腔注射地塞米松2 mg/只,日1次,连续5 d;对照组小鼠腹腔注射生理盐水0.2 ml/只。实验组每鼠经口感染107个人芽囊原虫,免疫抑制组和对照组每鼠经口灌服0.5 ml生理盐水。感染人芽囊原虫5 d后,剖杀小鼠,分别取十二指肠、空肠、回肠和结肠制作成组织切片,HE染色检查小鼠肠黏膜病变,免疫组化法检测各肠黏膜中IL-17和IL-23的表达水平。结果HE染色显示,肠道黏膜有不同程度炎症改变。IL-17和IL-23在实验组小鼠不同肠黏膜中的表达均显著高于免疫抑制剂组和对照组(P<0.05),而免疫抑制剂组与对照组IL-17和IL-23在小鼠不同肠黏膜中的表达差异无统计学意义(P>0.05)。IL-17在实验组小鼠十二指肠、空肠、结肠黏膜中的表达水平均显著高于回肠黏膜(P<0.05),IL-23在实验组小鼠十二指肠、空肠黏膜中的表达水平显著高于回肠和结肠黏膜(P<0.05)。结论IL-17和IL-23参与人芽囊原虫感染的免疫反应,且IL-17与IL-23在人芽囊原虫感染的免疫应答过程中有一定的相互调节作用。
Objective To observe the expression of interleukin 17 (IL-17) and IL-23 in intestinal tract of mice infected with Blastocystis hominis. Methods Thirty BABL / c mice were randomly divided into experimental group, immunosuppressant group and control group. The mice in experimental group and immunosuppressant group were injected intraperitoneally with dexamethasone 2 mg once daily for 5 d. The mice were injected intraperitoneally with 0.2 ml normal saline. In experimental group, 107 individuals of Blastocystis sp. Were inoculated into the mouth of mice in each experiment. Rats in immunosuppressive group and control group were orally administered with 0.5 ml of normal saline. After infected with Blastocystis, the mice were sacrificed and the duodenum, jejunum, ileum and colon were harvested to make tissue sections. The pathological changes of intestinal mucosa were observed by HE staining. The expressions of IL -17 and IL-23 expression levels. Results HE staining showed that intestinal mucosa with varying degrees of inflammatory changes. The expression of IL-17 and IL-23 in different intestinal mucosa of experimental mice were significantly higher than that of immunosuppressant and control groups (P <0.05), while the expression of IL-17 and IL-23 There was no significant difference in the expression of different intestinal mucosa in mice (P> 0.05). The expression level of IL-17 in the duodenum, jejunum and colon mucosa of experimental mice was significantly higher than that of the ileum mucosa (P <0.05). IL-23 expression in the duodenum and jejunum mucosa The expression level was significantly higher than ileum and colon mucosa (P <0.05). Conclusion IL-17 and IL-23 are involved in the immune response of Blastocystis infection, and IL-17 and IL-23 have a certain mutual regulation role in the immune response of Blastocystis infection.