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采用紫外-可见分光光度法测定了几种奶粉中的香兰素含量。在最大吸收波长435.0nm处,其吸光度与香兰素含量呈正比,线性方程为y=7.493 42x+0.001 3(r=0.999 7),线性范围为0.010 4～0.165 2g/L,加标回收率为98.6%,RSD为0.34%。该方法重现性好,适用于食品中香兰素含量的测定。
The vanillin content in several milk powders was determined by UV-Vis spectrophotometry. The absorbance at the maximum absorption wavelength of 435.0 nm was proportional to the content of vanillin, the linear equation was y = 7.493 42x + 0.001 3 (r = 0.999 7), and the linear range was 0.010 4 ~ 0.165 2 g / L. The recoveries Was 98.6% with a RSD of 0.34%. The method has good reproducibility and is suitable for the determination of vanillin content in food.