论文部分内容阅读
为获得糖尿病治疗的中药方剂,探讨由葛根、丹参、枳椇子组成的中药方剂对小鼠的抗糖尿病效应及潜在机制。利用链脲佐霉素(STZ)建立糖尿病模型,以空腹血糖大于11mmol/L为建模成功。30只小鼠分为空白组(健康小鼠)、试验组(糖尿病小鼠)和阴性对照组(糖尿病小鼠),每天对试验组进行中药方剂水煎服给药,空白组和阴性对照组每天灌服同等剂量蒸馏水,对小鼠的肝脏和肾脏生理指标变化进行检测。结果表明:STZ诱导的糖尿病小鼠给予葛根、丹参及枳椇子中药方剂灌服7d,试验组与阴性对照组相比,其空腹血糖降低,体重回升,肝脏指标白蛋白(ALB)下降31.58%,碱性磷酸酶(ALP)下降57.43%,谷丙转氨酶(ALT)回升87.80%,谷草转氨酶(AST)下降51.72%,总蛋白(TP)下降31.94%。由葛根、丹参和枳椇子组成的中药方剂在浓度为1g/L时对糖尿病小鼠有显著的治疗效果。
In order to obtain the prescription of traditional Chinese medicine for the treatment of diabetes mellitus, the antidiabetic effect and potential mechanism of traditional Chinese medicine prescription composed of Pueraria lobata, Radix Salviae Miltiorrhizae and Hovenia Dulcis Thunb on mice were explored. Streptozotocin (STZ) was used to establish a model of diabetes. The fasting blood glucose> 11mmol / L was modeled successfully. Thirty mice were divided into blank group (healthy mice), experimental group (diabetic mice) and negative control group (diabetic mice). The experimental groups were given Chinese herb prescription Shuijianbi daily, blank group and negative control group Dosing the same dose of distilled water daily, the mouse liver and kidney changes in physiological indicators were detected. The results showed that the administration of Pueraria root, Radix Salviae Miltiorrhizae and Hovenia Dulcis Thunb was given to the STZ-induced diabetic mice for 7 days. Compared with the negative control group, the fasting blood glucose, body weight and the index of albumin (ALB) decreased 31.58% , Alkaline phosphatase (ALP) decreased 57.43%, alanine aminotransferase (ALT) increased 87.80%, AST decreased 51.72%, total protein (TP) decreased 31.94%. From Pueraria, Salvia and Hovenia dulcis Thunb composition of traditional Chinese medicine prescription at a concentration of 1g / L on diabetic mice have a significant therapeutic effect.