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目的研究柴胡皂苷a(SSa)对谷氨酸(G lu)激活大鼠海马星形胶质细胞内Ca2+浓度([Ca2+]i)和白介素-6(IL-6)释放的影响。方法将原代培养并纯化的海马星形胶质细胞分为4组:对照组、L-G lu激活组(含L-G lu 0.1 mmol/L)和含L-G lu 0.1 mmol/L+SSa的药物干预组(SSa剂量分别为1.25 mg/L和0.625 mg/L),作用12 h后,运用Fluo3/Am荧光法测定星形胶质细胞内[Ca2+]i,ELISA法测细胞外液IL-6水平。结果G lu可显著升高星形胶质细胞[Ca2+]i(P<0.01),并使细胞外液IL-6水平显著增加(P<0.01);SSa能明显抑制G lu激活星形胶质细胞([Ca2+]i)的增加(P<0.01),并能降低G lu激活星形胶质细胞外液中IL-6的水平(P<0.01);其中1.25 mg/L剂量的SSa对G lu激活的星形胶质细胞细胞内[Ca2+]i升高和IL-6释放均有较好的抑制作用,与对照组比较,无显著性差异(P>0.05)。结论SSa可抑制G lu激活的星形胶质细胞[Ca2+]i升高和IL-6释放,这可能是其抗癫痫作用机制之一。
Objective To investigate the effects of saikosaponin a (SSa) on Ca2+ concentration ([Ca2+]i) and release of interleukin-6 (IL-6) in hippocampal astrocytes activated by glutamate (G lu). Methods Primary cultured and purified hippocampal astrocytes were divided into 4 groups: control group, LG lu activation group (containing LG lu 0.1 mmol/L) and drug intervention group containing LG lu 0.1 mmol/L+SSa ( The doses of SSa were 1.25 mg/L and 0.625 mg/L, respectively. After exposure for 12 h, [Ca2+]i in astrocytes was measured by Fluo3/Am fluorescence method. The level of IL-6 in the extracellular fluid was measured by ELISA. Results G lu significantly increased astrocytes [Ca 2+ ]i (P<0.01) and increased the level of extracellular fluid IL-6 (P<0.01). Ssa significantly inhibited G lu activation of astrocytes. Cellular ([Ca2+]i) increase (P<0.01), and G lu can reduce IL-6 levels in the activated astrocyte extracellular fluid (P<0.01); 1.25 mg/L SSA to G The increase of [Ca2+]i and the release of IL-6 in lu-activated astrocytes were well inhibited, and there was no significant difference compared with the control group (P>0.05). Conclusion Ssa can inhibit the increase of [Ca2+]i and IL-6 release in G lu-activated astrocytes, which may be one of the mechanisms of anti-epileptic effects.