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以平邑甜茶为实验材料,研究了干旱胁迫下线粒体交替氧化酶呼吸途径(AOX途径)对平邑甜茶叶片的光破坏防御作用。结果表明:干旱胁迫下线粒体AOX的蛋白表达量及AOX途径的相对活性均显著增加;强光下用水杨基羟肟酸(SHAM)抑制正常供水和干旱胁迫处理的植株的AOX途径后,正常供水的平邑甜茶叶片的PSII最大光化学效率(F_v/F_m)、快速叶绿素荧光响应曲线中的J点相对可变荧光(V_J)、PSII反应中心关闭的比例(1–qP)和光合线性电子传递速率(ETR)几乎不受影响,而干旱胁迫下的平邑甜茶叶片的ETR和F_v/F_m显著下降,1–q_P和V_J显著上升,这表明干旱胁迫下抑制AOX途径后,平邑甜茶叶片发生更严重的光抑制。上述结果表明:干旱胁迫下,AOX途径在平邑甜茶叶片的光破坏防御中起重要的作用。
The experiment was conducted to study the defensive effects of AOX pathway on the light damage of Malus hupehensis (Malpighiate) Brittle under drought stress. The results showed that under drought stress, the expression of mitochondrial AOX protein and the relative activity of AOX pathway were significantly increased. After salicylic hydroxamic acid (SHAM) inhibited the AOX pathway of plants treated by normal water supply and drought stress, the normal water supply , The PSII maximum photochemical efficiency (F_v / F_m), the J-point relative variable fluorescence (V_J), the ratio of PSII reaction center (1-qP) and the photosynthetic linear electron transfer rate in the rapid chlorophyll fluorescence response curve (ETR) were almost unaffected, whereas the ETR and F_v / F_m decreased significantly under drought stress, and the 1-q_P and V_J increased significantly, which indicated that the leaves of P. euphratica were more resistant to AOX after drought stress Severe photoinhibition. The above results show that under drought stress, AOX pathway plays an important role in the photodamage defense of P. euphratica leaves.