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在节瓜强雌系A43叶期和4叶期分别喷施赤霉素15mmol/L和蒸馏水后,对不同叶期茎尖的内源GA3、ABA、乙烯水平及真叶过氧化物酶活性变化测定,结果表明:赤霉素处理与蒸馏水处理相比,茎尖的内源GA3含量变化动态相似,至17叶期时两者含量相近;内源ABA含量变化动态相似,10叶期后,蒸馏水处理的内源ABA含量较高,ABA可能对花性转变影响不大;内源乙烯水平变化动态处理间有差异,3～4叶期是节瓜植株性别分化的关键时期,但此期赤霉素处理的内源乙烯水平低于蒸馏水处理,表明乙烯有促进节瓜雌性分化作用;蒸馏水处理真叶的过氧化物酶活性高于赤霉素处理,表明真叶的过氧化物酶活性对促进节瓜雌性分化有一定作用。
Changes of endogenous GA3, ABA, ethylene and true leaf peroxidase (APE) in different leaf stage shoot tips were induced by 15 mmol / L gibberellic acid and distilled water The results showed that GA3 content in shoot tip was similar to that in distilled water treatment, and the content of GA3 in shoot tip was similar at 17 leaf stage. The dynamic changes of endogenous ABA content were similar. After 10 leaves, distilled water ABA content was higher in ABA than that in ABA, ABA might have little effect on the transition of floret. There was a difference in dynamic changes of endogenous ethylene levels. The 3 ~ 4 leaf stage was the key period for sex differentiation of pumpkin. However, The level of endogenous ethylene in treated leaves was lower than that in distilled water, indicating that ethylene could promote the differentiation of female cucumbers. The activity of peroxidase in true leaves of distilled water was higher than that of GA3, indicating that the activity of peroxidase Section melon female differentiation has a certain effect.