论文部分内容阅读
【目的】对新疆小叶白蜡丛枝病植原体进行检测,通过其16S rRNA基因分析确定其分类地位。【方法】利用苯胺蓝和4′,6-二脒基-2-苯基吲哚(DAPI)染色,在荧光显微镜下观察新疆小叶白蜡嫩茎横切片;采用植原体16S rRNA基因的通用引物对P1/P7和R16F2n/R16R2进行直接和巢式PCR扩增,对得到的16S rRNA基因的序列进行RFLP和构建系统进化树分析。【结果】表现丛枝病症状的新疆小叶白蜡中存在植原体,暂命名为Fraxinus sogdianaBunge witches’broom phytoplasma(Fraxinus sogdianaBunge WB);其16S rRNA基因的序列GenBank登录号为KF061042,RFLP图谱与16Sr V-B亚组的枣疯病植原体相同,系统进化地位与枣疯病菌株AB052876相同。【结论】新疆小叶白蜡丛枝病植原体为16Sr V-B亚组成员。
【Objective】 The phytoplasma of Aspergillus Flavus in Xinjiang was detected by 16S rRNA gene analysis and its taxonomic status was determined. 【Method】 The transcripts of tender stems and leaves of C. albiflorus were observed under fluorescence microscope with aniline blue and 4 ’, 6-diamidino-2-phenylindole (DAPI) staining. A universal primer pair of phytoplasma 16S rRNA gene P1 / P7 and R16F2n / R16R2 for direct and nested PCR amplification. The obtained 16S rRNA gene sequences were analyzed by RFLP and phylogenetic tree analysis. 【Result】 Phytoplasma was present in the leaf wax of Xinjiang lobular. The sequence of 16S rRNA was GenBank accession number KF061042. The RFLP and 16Sr VB subgenotypes of Fraxinus sogdiana Bunge witches’broom phytoplasma (Fraxinus sogdiana Bunge WB) Group of jujube broom the same phytoplasma, phylogenetic position and jujube strain AB052876 the same. 【Conclusion】 The xylem of C. microphylla in Xinjiang is a 16Sr V-B subgroup.