论文部分内容阅读
目的:检测湖北地区HPV16阳性宫颈标本E2基因碳端(C)、铰链区(H)、氮端(N)的缺失状态,探讨其与CIN及宫颈癌的关系。方法:将122例宫颈标本按病变程度分为宫颈癌组、CINⅡ~Ⅲ组、对照组。应用多重聚合酶链反应(PCR)将各组HPV16感染标本E2基因的C、H、N分别与E6基因同时扩增,检测E2基因各区域的缺失状态,并应用Scion Image4.0软件半定量分析E2、E6基因条带灰度值,判断HPV DNA的整合状态。结果:宫颈癌组E2基因C、H、N缺失率分别是22.5%,50%,77.5%;CIN组为30.77%,69.23%,92.31%;对照组为9.09%,27.27%,27.27%。E2基因C、H缺失率在3组的任两组间比较无统计学差异(P>0.05),而E2基因N端缺失率在宫颈癌组与对照组之间及CIN组与对照组之间差异有统计学意义(P均<0.01)。结论:HPV16病毒E2基因N端缺失状态与CIN及宫颈癌有内在关系。在检测HPV感染同时检测E2基因N端缺失状态,对间接判断预后及指导后续治疗有重要意义。
Objective: To detect the deletion of E2, C (hinge) and N (N) in E2 gene of HPV16-positive cervical specimens in Hubei Province, and to explore its relationship with CIN and cervical cancer. Methods: 122 cases of cervical specimens were divided into cervical cancer group, CIN Ⅱ ~ Ⅲ group and control group according to the degree of lesion. Multiplex polymerase chain reaction (PCR) was used to amplify C, H and N of E2 gene of each group of HPV16 infection simultaneously with E6 gene to detect the deletion status of each region of E2 gene. Semi-quantitative analysis was performed by using Scion Image 4.0 software E2, E6 gene band gray value to determine the integration of HPV DNA status. Results: The deletion rates of E2, C, H and N in cervical cancer group were 22.5%, 50% and 77.5% respectively. The CIN group was 30.77%, 69.23% and 92.31% respectively. The control group was 9.09%, 27.27% and 27.27% respectively. There was no significant difference in the C, H deletion rate of E2 between the two groups (P> 0.05), while the deletion of E2 gene between cervical cancer group and control group and between CIN group and control group The difference was statistically significant (P <0.01). Conclusion: The N-terminal deletion of E2 gene of HPV16 virus is intrinsically related to CIN and cervical cancer. Detection of HPV infection at the same time detection of E2 gene N-terminal deletion status, indirect prognosis and follow-up treatment of great significance.