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为开发新型木荷皂甙杀稻瘟剂,分别采用菌丝生长速率法、显微观察、电导法和比色法研究木荷活性皂甙对稻瘟病菌Magnaporthe oryzae(Hebert)Barr.的活性,以及对病原细胞形态及其胞外生理生化成分含量的影响。结果显示:木荷皂甙M2IGR5抗稻瘟病菌的EC50为16.41μg/mL、EC90为76.16μg/mL;其EC50和EC90处理稻瘟病菌菌丝体24 h后,可导致菌丝断裂、细胞肿胀;处理去壁的原生质体2 h即可导致其变型、粘连、破裂,引起病原菌菌丝细胞内含物大量渗漏;处理2 h后的可溶性蛋白渗漏量和可溶性总糖分别较对照高10倍和30~60倍,且处理液的电导率升高、几丁质酶活性增强、胞壁几丁质组成成分N-乙酰葡萄糖胺脱落。研究表明,木荷皂甙抗稻瘟病菌不仅导致膜通透性增大、完整性丧失,而且还间接引起细胞壁降解,加速了稻瘟病菌细胞的凋亡。
In order to develop a new type of mulikosaponin, the activity of Magnaporthe oryzae (Hebert) Barr. Was investigated by mycelial growth rate, microscopic observation, conductivity and colorimetric methods, respectively. Influence of Pathogen Morphology and Extracellular Physiological and Biochemical Content. The results showed that the EC50 of M. sagittatum M2IGR5 against M. grisea was 16.41μg / mL and the EC90 was 76.16μg / mL. The EC50 and EC90 treatment of M. grisea mycelia for 24 h resulted in hyphae rupture and cell swelling. Treatment of the protoplast of the desquamated wall for 2 h resulted in a large amount of leakage of the mycelium of the pathogenic bacteria, resulting in a large amount of leakage of the mycelium of the pathogenic bacteria. The amount of soluble protein leakage and the total soluble sugar after the treatment for 2 h were 10 times higher than that of the control And 30 to 60 times, and the conductivity of the treatment solution increased, chitinase activity increased, cell wall chitin composition N-acetylglucosamine shed. The results showed that the saponins from Magnaporthe grisea not only caused the increase of membrane permeability and the loss of integrity, but also caused the cell wall degradation indirectly and accelerated the apoptosis of the rice blast fungus.