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目的:探讨异丙酚对大鼠内毒素脑损伤中磷酸化JAK2、STAT3蛋白表达的影响。方法:健康清洁级SD大鼠72只,雌雄不限,体质量220～250 g,随机分为3组(n=24):L组(内毒素组)和LP组(内毒素+异丙酚组)经颈内动脉注射内毒素200μg建立大鼠感染性脑损伤模型,C组(对照组)经颈内动脉注射等量生理盐水,LP组颈内动脉注射内毒素后即予异丙酚100 mg.kg-1剂量腹腔注射。3组分别于6,12,24,48 h随机处死6只大鼠,取额叶皮质,检测脑组织含水量、磷酸化JAK2和STAT3蛋白表达水平,光镜下观察脑组织形态及病理变化。结果:与C组相比,L组、LP组各时间点脑组织含水量、P-JAK2和P-STAT3表达增加(P<0.05,P<0.01);与L组比较,LP组各时间点脑含水量、P-JAK2和P-STAT3表达明显减少(P<0.05,P<0.01)。结论:异丙酚减轻大鼠内毒素性脑损伤的机制可能与抑制脑组织磷酸化JAK2、STAT3表达水平上调有关。
Objective: To investigate the effect of propofol on the expression of phosphorylated JAK2 and STAT3 in endotoxin-induced brain injury in rats. Methods: Seventy two healthy SD rats were randomly divided into 3 groups (n = 24): L group (endotoxin group) and LP group (endotoxin + propofol group) Group) was injected intracarotidly with 200μg of endotoxin to establish the model of infectious brain injury in rats. The rats in group C (control group) were injected with the same amount of saline through the internal carotid artery. After the injection of endotoxin in the carotid artery of group LP, propofol 100 mg.kg-1 dose intraperitoneal injection. Six rats were randomly sacrificed at 6, 12, 24, and 48 h after operation. The cortex of the frontal lobe was taken and the brain water content, the expression of phosphorylated JAK2 and STAT3 protein were measured. The morphological and pathological changes of brain were observed under light microscope. Results Compared with group C, the water content of brain tissue and the expression of P-JAK2 and P-STAT3 in L group and LP group increased at each time point (P <0.05, P <0.01). Compared with L group, LP group at each time point Brain water content, P-JAK2 and P-STAT3 expression was significantly reduced (P <0.05, P <0.01). Conclusion: The mechanism of propofol in attenuating endotoxin-induced brain injury in rats may be related to the up-regulation of phosphorylation of JAK2 and STAT3 in brain tissue.