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为探讨He-Ne激光对增强UV-B辐射造成的小麦“翘根”的影响,采用He-Ne激光(辐照剂量为5mW·mm-2)辐照,对增强UV-B辐射(辐射剂量为10.08kJ·m-2·d-1)条件下小麦根部肌动蛋白含量、黄酮类化合物含量及种类变化进行研究。结果表明,增强UV-B辐射后小麦主胚根长变短,肌动蛋白及黄酮类化合物含量降低,而He-Ne激光组差异不明显;两种辐射的复合处理组结果低于CK组、高于UV-B处理组,“翘根”现象得到缓解;不同处理组的黄酮类化合物扩散速度不同,UV-B组黄酮类化合物种类发生变化。肌动蛋白及黄酮类含量变化是增强UV-B辐射后小麦“翘根”现象产生的原因之一,He-Ne激光对UV-B辐射造成的小麦“翘根”损伤具有一定的修复作用。
In order to investigate the effect of He-Ne laser on the wheat root growth induced by enhanced UV-B radiation, He-Ne laser (5 mW · mm-2 irradiation dose) Radiation dose 10.08kJ · m-2 · d-1) under the conditions of wheat root actin content, flavonoids content and species changes were studied. The results showed that the main radicle length of wheat was shorter and the content of actin and flavonoids decreased after UV-B irradiation, but the difference was not obvious in He-Ne laser group. The results of the two radiation treatments were lower than CK group, Higher than UV-B treatment group, “Alice” phenomenon was alleviated; different treatment groups of flavonoids diffusion speed, UV-B group flavonoids species changes. The changes of actin and flavonoid content were one of the reasons for the increase of UV-B radiation in wheat after “UV-B radiation”. The He-Ne laser had a definite effect on the damage of wheat caused by UV-B radiation Repair effect.