目的:探讨苦参碱对巨细胞病毒(Cytomegalovirus,CMV)感染小鼠重要脏器肝、肺组织中鼠巨细胞病毒(MCMV)-DNA载量的影响。方法:建立全身播散性CMV感染小鼠模型,随机分为模型对照组、苦参碱治疗组和更昔洛韦治疗组,另设正常对照组,每组32只。采用荧光定量PCR方法检测各组小鼠肝、肺组织中MCMV-DNA载量并比较其变化。结果:正常对照组小鼠肝、肺组织中MCMV-DNA检测呈阴性,其余三组于接种病毒后第3天可以检测到较高水平MCMV-DNA,第7天达高峰。第14天肝组织病毒载量比较,更昔洛韦治疗组<苦参碱治疗组<模型对照组,差异有统计学意义(F=63.10,P<0.01);肺组织病毒载量变化趋势同肝组织病毒载量,第14天肺组织病毒载量比较,更昔洛韦治疗组<苦参碱治疗组<模型对照组,差异有统计学意义(F=194.88,P<0.01)。结论:苦参碱可以降低CMV感染小鼠肝、肺组织MCMV-DNA载量,具有抗MCMV作用。 Objective: To investigate the effect of matrine on cytomegalovirus (MCMV) -DNA load in liver and lung tissues of mice infected with cytomegalovirus (CMV). Methods: The mice model of systemic disseminated CMV infection was established and randomly divided into model control group, matrine treatment group and ganciclovir treatment group, another normal control group, 32 in each group. The quantity of MCMV-DNA in the liver and lung tissues of mice in each group was detected by real-time quantitative PCR and compared with the changes. Results: MCMV-DNA in the liver and lung tissue of the normal control group was negative. The other three groups could detect high level of MCMV-DNA on the third day after virus inoculation, reaching the peak on the seventh day. On the 14th day, the viral load of liver tissue was significantly higher in the ganciclovir-treated group