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目的:观察在K562细胞高表达miR-10a对细胞增殖及凋亡的影响。方法:用pAd-pre-miR-10a重组腺病毒载体感染K562细胞,采用RT-PCR检测感染后K562细胞中miR-10a和bcr/abl融合基因的表达水平,Western blot法检测BCR/ABL融合蛋白表达水平,MTT、流式细胞术(FCM)分别检测感染后K562细胞的增殖及凋亡。结果:与对照组相比,K562细胞在转染pAd-pre-miR-10a重组腺病毒载体后,其miR-10a表达水平明显升高、bcr/abl融合基因水平显著降低;BCR/ABL融合蛋白表达明显下调、K562细胞增殖活力被抑制、细胞凋亡增加,差异均具有统计学意义(P<0.05)。结论:pAd-pre-miR-10a重组腺病毒载体能明显抑制K562细胞的增殖、促进细胞凋亡。
AIM: To observe the effect of miR-10a overexpression on cell proliferation and apoptosis in K562 cells. Methods: The K562 cells were infected with pAd-pre-miR-10a recombinant adenovirus vector and the expression of miR-10a and bcr / abl fusion genes in K562 cells was detected by RT-PCR. The expression of BCR / ABL fusion protein The expression and apoptosis of K562 cells were detected by MTT assay and flow cytometry (FCM). Results: Compared with the control group, the expression of miR-10a and the bcr / abl fusion gene were significantly decreased in K562 cells transfected with pAd-pre-miR-10a recombinant adenovirus vector; The expression of K562 was significantly down-regulated. The proliferation of K562 cells was inhibited and the apoptosis of K562 cells was increased. The differences were statistically significant (P <0.05). Conclusion: Recombinant adenovirus vector pAd-pre-miR-10a can significantly inhibit the proliferation of K562 cells and promote apoptosis.